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Title: Development of pure certified reference material of stevioside
Authors: Nongluck Tangpaisarnkul
Patoomratana Tuchinda
Prapin Wilairat
Atitaya Siripinyanond
Juwadee Shiowattana
Saksit Nobsathian
Mahidol University
Keywords: Agricultural and Biological Sciences;Chemistry
Issue Date: 30-Jul-2018
Citation: Food Chemistry. Vol.255, (2018), 75-80
Abstract: © 2018 Elsevier Ltd Pure stevioside was extracted from leaves of Stevia rebaudiana (Bertoni) to be used as a reference material in the instrument calibration or method validation process. The mass fraction was determined by comparison between the mass balance method and quantitative nuclear magnetic resonance (qNMR) spectroscopy. The impurities in the sample were analyzed by Karl Fischer titration for moisture content and thermogravimetric analysis for inorganic residue. Homogeneity, together with short term and long term stability, were also studied and the uncertainty was reported. The certified value of this mass fraction is 0.986 ± 0.0019 (k = 2) with 1 year stability.
ISSN: 18737072
Appears in Collections:Scopus 2018

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