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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/44721
Title: Gene expression and promoter characterization of heat-shock protein 90B gene (HSP90B) in the model unicellular green alga Chlamydomonas reinhardtii
Authors: Somchoke Traewachiwiphak
Chotika Yokthongwattana
Parthompong Ves-Urai
Varodom Charoensawan
Kittisak Yokthongwattana
Kasetsart University
Mahidol University
Keywords: Agricultural and Biological Sciences;Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jul-2018
Citation: Plant Science. Vol.272, (2018), 107-116
Abstract: © 2018 Elsevier B.V. Molecular chaperones or heat shock proteins are a large protein family with important functions in every cellular organism. Among all types of the heat shock proteins, information on the ER-localized HSP90 protein (HSP90B) and its encoding gene is relatively scarce in the literature, especially in photosynthetic organisms. In this study, expression profiles as well as promoter sequence of the HSP90B gene were investigated in the model green alga Chlamydomonas reinhardtii. We have found that HSP90B is strongly induced by heat and ER stresses, while other short-term exposure to abiotic stresses, such as salinity, dark-to-light transition or light stress does not appear to affect the expression. Promoter truncation analysis as well as chromatin immunoprecipitation using the antibodies recognizing histone H3 and acetylated histone H3, revealed a putative core constitutive promoter sequence between −1 to −253 bp from the transcription start site. Our results also suggested that the nucleotides upstream of the core promoter may contain repressive elements such as putative repressor binding site(s).
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85046372480&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/44721
ISSN: 18732259
01689452
Appears in Collections:Scopus 2018

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