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|Title:||Sensory acceptable equivalent doses of β-phenylethyl isothiocyanate (PEITC) induce cell cycle arrest and retard the growth of p53 mutated oral cancer in vitro and in vivo|
Alison Lea Fitzgerald
Jeffrey N. Myers
Sun Yat-Sen University Cancer Center
Sun Yat-Sen University, Zhongshan School of Medicine
Sun Yat-Sen University
University of Texas MD Anderson Cancer Center
The Dental Innovation Foundation under Royal Patronage
|Keywords:||Agricultural and Biological Sciences|
|Citation:||Food and Function. Vol.9, No.7 (2018), 3640-3656|
|Abstract:||© The Royal Society of Chemistry. High doses of β-phenylethyl isothiocyanate (PEITC), a phytochemical in cruciferous vegetables, are not feasible for consumption due to a strong mouth-tingling effect. This study investigated the anti-cancer effect of PEITC at sensory acceptable doses. In vitro, PEITC was selectively toxic to oral cancer cells (CAL-27, FaDu, SCC4, SCC 9, SCC15, SCC25 and TU138), compared to oral keratinocytes (OKF6/TERT2 and NOK/Si). In vivo, 5 and 10 mg kg -1 PEITC, equivalent to human organoleptically acceptable doses, retarded tumor growth and prolonged the survival of mice bearing p53-mutated oral cancer cells-TU138 xenograft. Mechanistically, PEITC induced ROS accumulation, nuclear translocation of p53 and p21 and G1/S cell cycle arrest in vitro; increased p53 and 8-oxo-dG levels; and decreased Ki-67 intense/mild staining ratios without TUNEL changes in vivo. These findings suggested that the sensory acceptable doses of PEITC selectively induced ROS-mediated cell cycle arrest leading to delayed tumor progression and extended survival. PEITC could be a functional ingredient for oral cancer prevention.|
|Appears in Collections:||Scopus 2018|
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