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dc.contributor.authorMongkon Charoenpitakchaien_US
dc.contributor.authorKulachet Wiwatwarayosen_US
dc.contributor.authorNattapon Jaisupaen_US
dc.contributor.authorMuhamad Rusdi Ahmad Rusmilien_US
dc.contributor.authorSupachoke Mangmoolen_US
dc.contributor.authorWayne C. Hodgsonen_US
dc.contributor.authorChetana Ruangpratheepen_US
dc.contributor.authorLawan Chanhomeen_US
dc.contributor.authorJaneyuth Chaisakulen_US
dc.contributor.otherThai Red Cross Agencyen_US
dc.contributor.otherArmy Institute of Pathologyen_US
dc.contributor.otherMonash Universityen_US
dc.contributor.otherInternational Islamic University Malaysiaen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherPhramongkutklao College of Medicineen_US
dc.date.accessioned2019-08-23T10:18:37Z-
dc.date.available2019-08-23T10:18:37Z-
dc.date.issued2018-03-09en_US
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Vol.24, No.1 (2018)en_US
dc.identifier.issn16789199en_US
dc.identifier.issn16789180en_US
dc.identifier.other2-s2.0-85043452788en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85043452788&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/44797-
dc.description.abstract© 2018 The Author(s). Background: Envenoming by kraits (genus Bungarus) is a medically significant issue in South Asia and Southeast Asia. Malayan krait (Bungarus candidus) venom is known to contain highly potent neurotoxins. In recent years, there have been reports on the non-neurotoxic activities of krait venom that include myotoxicity and nephrotoxicity. However, research on such non-neurotoxicity activities of Malayan krait venom is extremely limited. Thus, the aim of the present study was to determine the myotoxic, cytotoxic and nephrotoxic activities of B. candidus venoms from northeastern (BC-NE) and southern (BC-S) Thailand in experimentally envenomed rats. Methods: Rats were administered Malayan krait (BC-NE or BC-S) venom (50 μg/kg, i.m.) or 0.9% NaCl solution (50 μL, i.m.) into the right hind limb. The animals were sacrificed 3, 6 and 24 h after venom administration. The right gastrocnemius muscle and both kidneys were collected for histopathological analysis. Blood samples were also taken for determination of creatine kinase (CK) and lactate dehydrogenase (LDH) levels. The human embryonic kidney cell line (HEK-293) was used in a cell proliferation assay to determine cytotoxic activity. Results: Administration of BC-NE or BC-S venom (50 μg/kg, i.m.) caused time-dependent myotoxicity, characterized by an elevation of CK and LDH levels. Histopathological examination of skeletal muscle displayed marked muscle necrosis and myofiber disintegration 24 h following venom administration. Both Malayan krait venoms also induced extensive renal tubular injury with glomerular and interstitial congestion in rats. BC-NE and BC-S venoms (100-0.2 μg/mL) caused concentration-dependent cytotoxicity on the HEK-293 cell line. However, BC-NE venom (IC50 = 8 ± 1 μg/mL; at 24 h incubation; n = 4) was found to be significantly more cytotoxic than BC-S venom (IC50 = 15 ± 2 μg/mL; at 24 h incubation; n = 4). In addition, the PLA2 activity of BC-NE venom was significantly higher than that of BC-S venom. Conclusions: This study found that Malayan krait venoms from both populations possess myotoxic, cytotoxic and nephrotoxic activities. These findings may aid in clinical diagnosis and treatment of envenomed patients in the future.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85043452788&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectImmunology and Microbiologyen_US
dc.subjectMedicineen_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleNon-neurotoxic activity of Malayan krait (Bungarus candidus) venom from Thailanden_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1186/s40409-018-0146-yen_US
Appears in Collections:Scopus 2018

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