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dc.contributor.authorDian Anggraini Surotoen_US
dc.contributor.authorShigeru Kitanien_US
dc.contributor.authorMasayoshi Araien_US
dc.contributor.authorHaruo Ikedaen_US
dc.contributor.authorTakuya Nihiraen_US
dc.contributor.otherOsaka Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherKitasato Universityen_US
dc.date.accessioned2019-08-23T10:22:07Z-
dc.date.available2019-08-23T10:22:07Z-
dc.date.issued2018-01-01en_US
dc.identifier.citationPLoS ONE. Vol.13, No.1 (2018)en_US
dc.identifier.issn19326203en_US
dc.identifier.other2-s2.0-85040347904en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85040347904&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/44897-
dc.description.abstract© 2018 Suroto et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Phthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as a cryptic metabolite of Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data of S. avermitilis is publicly available, no plausible biosynthetic gene cluster for phthoxazolin A is apparent in the sequence data. Here, we identified and characterized the phthoxazolin A (ptx) biosynthetic gene cluster through genome sequencing, comparative genomic analysis, and gene disruption. Sequence analysis uncovered that the putative ptx biosynthetic genes are laid on an extra genomic region that is not found in the public database, and 8 open reading frames in the extra genomic region could be assigned roles in the biosynthesis of the oxazole ring, triene polyketide and carbamoyl moieties. Disruption of the ptxA gene encoding a discrete acyltransferase resulted in a complete loss of phthoxazolin A production, confirming that the trans-AT type I PKS system is responsible for the phthoxazolin A biosynthesis. Based on the predicted functional domains in the ptx assembly line, we propose the biosynthetic pathway of phthoxazolin A.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85040347904&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleCharacterization of the biosynthetic gene cluster for cryptic phthoxazolin a in streptomyces avermitilisen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1371/journal.pone.0190973en_US
Appears in Collections:Scopus 2018

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