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Title: Complement susceptibility in relation to genome sequence of recent Klebsiella pneumoniae isolates from Thai hospitals
Authors: Jessica Loraine
Eva Heinz
Jessica de Sousa Almeida
Oleksandr Milevskyy
Supayang P. Voravuthikunchai
Potjanee Srimanote
Pattarachai Kiratisin
Nicholas R. Thomson
Peter W. Taylor
London School of Hygiene & Tropical Medicine
UCL School of Pharmacy
Thammasat University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Prince of Songkla University
Wellcome Sanger Institute
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology
Issue Date: 1-Nov-2018
Citation: mSphere. Vol.3, No.6 (2018)
Abstract: © 2018 Loraine et al. The capacity to resist the bactericidal action of complement (C') is a strong but poorly understood virulence trait in Klebsiella spp. Killing requires activation of one or more C' pathways, assembly of C5b-9 membrane attack complexes (MACs) on the surface of the outer membrane (OM), and penetration of MACs into the target bilayer. We interrogated whole-genome sequences of 164 Klebsiella isolates from three tertiary hospitals in Thailand for genes encoding surface-located macromolecules considered to play a role in determination of C' resistance. Most isolates (154/164) were identified as Klebsiella pneumoniae, and the collection conformed to previously established population structures and antibiotic resistance patterns. The distribution of sequence types (STs) and capsular (K) types were also typical of global populations. The majority (64%) of isolates were resistant to C', and the remainder were either rapidly or slowly killed. All isolates carried genes encoding capsular polysaccharides (K antigens), which have been strongly linked to C' resistance. In contrast to previous reports, there were no differences in the amount of capsule produced by C'-resistant isolates compared to C'-susceptible isolates, nor was there any correlation between serum reactivity and the presence of hypermucoviscous capsules. Similarly, there were no correlations between the presence of genes specifying lipopolysaccharide O-side chains or major OM proteins. Some virulence factors were found more frequently in C'-resistant isolates but were considered to reflect clonal ST expansion. Thus, no single gene accounts for the C' resistance of the isolates sequenced in this study.
ISSN: 23795042
Appears in Collections:Scopus 2018

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