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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/45053
Title: Thermoresponsive Bacteriophage Nanocarrier as a Gene Delivery Vector Targeted to the Gastrointestinal Tract
Authors: Katawut Namdee
Mattaka Khongkow
Suwimon Boonrungsiman
Naiyaphat Nittayasut
Paladd Asavarut
Sasithon Temisak
Nattika Saengkrit
Satit Puttipipatkhachorn
Amin Hajitou
Kiat Ruxrungtham
Teerapong Yata
Chulalongkorn University
Imperial College London
Mahidol University
Thailand National Science and Technology Development Agency
National Institute of Metrology (Thailand) (NIMT)
Keywords: Biochemistry, Genetics and Molecular Biology;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 7-Sep-2018
Citation: Molecular Therapy - Nucleic Acids. Vol.12, (2018), 33-44
Abstract: © 2018 The Author(s) The use of the gastrointestinal tract as a site for the local delivery of DNA is an exciting prospect. In order to obtain an effective vector capable of delivering a gene of interest to target cells to achieve sufficient and sustained transgene expression, with minimal toxicity, we developed a new generation of filamentous bacteriophage. This particular bacteriophage was genetically engineered to display an arginine-glycine-aspartic acid (RGD) motif (an integrin-binding peptide) on the major coat protein pVIII and carry a mammalian DNA cassette. One unanticipated observation is the thermoresponsive behavior of engineered bacteriophage. This finding has led us to simplify the isolation method to purify bacteriophage particles from cell culture supernatant by low-temperature precipitation. Our results showed that, in contrast to non-surface modified, the RGD-modified bacteriophage was successfully used to deliver a transgene to mammalian cells. Our in vitro model of the human intestinal follicle-associated epithelium also demonstrated that bacteriophage particles were stable in simulated gastrointestinal fluids and able to cross the human intestinal barrier. In addition, we confirmed an adjuvant property of the engineered bacteriophage to induce nitric oxide production by macrophages. In conclusion, our study demonstrated the possibility of using bacteriophage for gene transfer in the gastrointestinal tract.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85047075497&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/45053
ISSN: 21622531
Appears in Collections:Scopus 2018

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