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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/45095
Title: Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
Authors: Bootsakorn Boonkaew
Lunnathaya Tapeng
Ratchapong Netsrithong
Chinnavuth Vatanashevanopakorn
Kovit Pattanapanyasat
Methichit Wattanapanitch
Silpakorn University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Aug-2018
Citation: Stem Cell Research. Vol.31, (2018), 79-82
Abstract: © 2018 The Authors In this study, we used hair follicle keratinocytes for reprogramming. Collection of plucked hairs offers advantages over other somatic cells because no medical professional or operation room is required. Keratinocytes were isolated from plucked hairs of a 21-year-old healthy woman and characterized for the expression of cytokeratin 14 (CK14). Reprogramming of keratinocytes was performed using Sendai virus. Further characterization of the keratinocyte-derived iPSC line (designated as MUSIi006-A) confirmed that the cell line was pluripotent, free from Sendai viral genome and transgenes, and retained normal karyotype. Our method represents an easy, non-invasive and efficient approach for iPSC generation from hair samples.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85050001250&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/45095
ISSN: 18767753
18735061
Appears in Collections:Scopus 2018

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