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Title: | Establishment of an integration-free induced pluripotent stem cell line (MUSIi005-A) from exfoliated renal epithelial cells |
Authors: | Bootsakorn Boonkaew Weeradee Thummavichit Ratchapong Netsrithong Chinnavuth Vatanashevanopakorn Kovit Pattanapanyasat Methichit Wattanapanitch Silpakorn University Faculty of Medicine, Siriraj Hospital, Mahidol University |
Keywords: | Biochemistry, Genetics and Molecular Biology |
Issue Date: | 1-Jul-2018 |
Citation: | Stem Cell Research. Vol.30, (2018), 34-37 |
Abstract: | © 2018 The Authors Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29. Our protocol offers a non-invasive and efficient approach for iPSC generation from patients with genetic or acquired disorders. |
URI: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85047008508&origin=inward http://repository.li.mahidol.ac.th/dspace/handle/123456789/45114 |
ISSN: | 18767753 18735061 |
Appears in Collections: | Scopus 2018 |
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