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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/45192
Title: Differentiation of stem cells from human deciduous and permanent teeth into spiral ganglion neuron-like cells
Authors: Thanasup Gonmanee
Charoensri Thonabulsombat
Kutkao Vongsavan
Hathaitip Sritanaudomchai
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Dentistry;Medicine
Issue Date: 1-Apr-2018
Citation: Archives of Oral Biology. Vol.88, (2018), 34-41
Abstract: © 2018 Elsevier Ltd Objective: Stem cells from pulp tissue are a promising cell-based therapy for neurodegenerative patients based on their origin in the neural crest. The aim of this study was to differentiate and evaluate the ability of human dental pulp stem cells from permanent teeth (DPSC) and stem cells from human exfoliated deciduous teeth (SHED) to differentiate into spiral ganglion neurons. Design: After isolation and characterization of mesenchymal stem cell properties, DPSC and SHED were treated with the neurotrophins brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and glial cell-derived neurotrophic factor (GDNF). The differentiation was identified by immunostaining and qRT-PCR analysis of neuronal markers and measuring intracellular calcium activity. Results: After 2 weeks of induction, morphological changes were observed in both DPSC and SHED. The differentiated cells expressed neuron-specific class III beta-tubulin, GATA binding protein 3 (GATA3) and tropomyosin receptor kinase B, protein markers of spiral ganglion neurons. These cells also showed upregulation of the genes encoding these proteins, namely GATA3 and neurotrophic receptor tyrosine kinase 2. Intracellular calcium dynamics that reflect neurotransmitter release were observed in differentiated DPSC and SHED. Conclusion: These results demonstrate that dental pulp stem cells from permanent and deciduous teeth can differentiate into spiral ganglion neuron-like cells.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85041515024&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/45192
ISSN: 18791506
00039969
Appears in Collections:Scopus 2018

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