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|Title:||Paper-based immunosensor with signal amplification by enzyme-labeled anti-p16 <sup>INK4a</sup> multifunctionalized gold nanoparticles for cervical cancer screening|
Thailand National Nanotechnology Center
Faculty of Medicine, Siriraj Hospital, Mahidol University
|Keywords:||Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Engineering;Materials Science;Medicine;Pharmacology, Toxicology and Pharmaceutics|
|Citation:||Nanomedicine: Nanotechnology, Biology, and Medicine. Vol.14, No.3 (2018), 1051-1058|
|Abstract:||© 2018 Elsevier Inc. The aim of this study was to develop a paper-based immunosensor for cervical cancer screening, with signal amplification by multifunctionalized gold nanoparticles (AuNPs). The AuNPs were functionalized with a highly specific antibody to the p16 INK4a cancer biomarker. The signal was amplified using a combination of the peroxidase activity of horseradish peroxidase (HRP) enzyme-antibody conjugate and the peroxidase-like activity of the AuNPs. The immune complex of p16 INK4a protein and multifunctionalized AuNPs was deposited on the nitrocellulose membrane, and a positive result was generated by catalytic oxidation of peroxidase enzyme substrate 3,3’,5,5’-Tetramethylbenzidine (TMB). The entire reaction occurred on the membrane within 30 min. Evaluation in clinical samples revealed 85.2% accuracy with a kappa coefficient of 0.69. This proof of concept study demonstrates the successful development of a highly accurate, paper-based immunosensor that is easy to interpret using the naked eye and that is suitable for cervical cancer screening in low-resource settings.|
|Appears in Collections:||Scopus 2018|
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