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|Title:||Oncogenic RAS-Induced perinuclear signaling complexes requiring KSR1 regulate signal transmission to downstream targets|
|Authors:||Sandip K. Basu|
Christopher J. Westlake
Deborah K. Morrison
Peter F. Johnson
National Center for Toxicological Research
National Cancer Institute at Frederick
|Keywords:||Biochemistry, Genetics and Molecular Biology;Medicine|
|Citation:||Cancer Research. Vol.78, No.4 (2018), 891-908|
|Abstract:||© 2017 American Association for Cancer Research. The precise characteristics that distinguish normal and oncogenic RAS signaling remain obscure. Here, we show that oncogenic RAS and BRAF induce perinuclear relocalization of several RAS pathway proteins, including the kinases CK2 and p- ERK1/2 and the signaling scaffold KSR1. This spatial reorganization requires endocytosis, the kinase activities of MEK-ERK and CK2, and the presence of KSR1. CK2a colocalizes with KSR1 and Rab11, a marker of recycling endosomes, whereas p- ERK associates predominantly with a distinct KSR1-positive endosomal population. Notably, these perinuclear signaling complexes (PSC) are present in tumor cell lines, mouse lung tumors, and mouse embryonic fibroblasts undergoing RAS-induced senescence. PSCs are also transiently induced by growth factors (GF) in nontransformed cells with delayed kinetics (4–6 hours), establishing a novel late phase of GF signaling that appears to be constitutively activated in tumor cells. PSCs provide an essential platform for RAS-induced phosphorylation and activation of the prosenescence transcription factor C/EBPb in primary MEFs undergoing senescence. Conversely, in tumor cells, C/EBPb activation is suppressed by 30UTR-mediated localization of Cebpb transcripts to a peripheral cytoplasmic domain distinct from the PSC region. Collectively, our findings indicate that sustained PSC formation is a critical feature of oncogenic RAS/BRAF signaling in cancer cells that controls signal transmission to downstream targets by regulating selective access of effector kinases to substrates such as C/EBPb. Significance: In addressing the long-standing question of the difference between normal and oncogenic RAS pathway signaling, this study shows that oncogenic RAS specifically triggers constitutive endocytosis-dependent movement of effector kinases to a perinuclear region, thereby creating connections to unique downstream targets such as the core prosenescence and the inflammatory regulatory transcription factor C/EBPb.|
|Appears in Collections:||Scopus 2018|
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