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Title: Discovery of a new diol-containing polyketide by heterologous expression of a silent biosynthetic gene cluster from Streptomyces lavendulae FRI-5
Authors: Ivy Grace Umadhay Pait
Shigeru Kitani
Farah Wahidah Roslan
Dana Ulanova
Masayoshi Arai
Haruo Ikeda
Takuya Nihira
Osaka University
Kochi University
Mahidol University
Kitasato University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Immunology and Microbiology
Issue Date: 1-Feb-2018
Citation: Journal of Industrial Microbiology and Biotechnology. Vol.45, No.2 (2018), 77-87
Abstract: © 2017, Society for Industrial Microbiology and Biotechnology. The genome of streptomycetes has the ability to produce many novel and potentially useful bioactive compounds, but most of which are not produced under standard laboratory cultivation conditions and are referred to as silent/cryptic secondary metabolites. Streptomyces lavendulae FRI-5 produces several types of bioactive compounds. However, this strain may also have the potential to biosynthesize more useful secondary metabolites. Here, we activated a silent biosynthetic gene cluster of an uncharacterized compound from S. lavendulae FRI-5 using heterologous expression. The engineered strain carrying the silent gene cluster produced compound 5, which was undetectable in the culture broth of S. lavendulae FRI-5. Using various spectroscopic analyses, we elucidated the chemical structure of compound 5 (named lavendiol) as a new diol-containing polyketide. The proposed assembly line of lavendiol shows a unique biosynthetic mechanism for polyketide compounds. The results of this study suggest the possibility of discovering more silent useful compounds from streptomycetes by genome mining and heterologous expression.
ISSN: 14765535
Appears in Collections:Scopus 2018

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