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Title: Determination of irinotecan, SN-38 and SN-38 glucuronide using HPLC/MS/MS: Application in a clinical pharmacokinetic and personalized medicine in colorectal cancer patients
Authors: Chalirmporn Atasilp
Pichai Chansriwong
Ekapob Sirachainan
Thanyanan Reungwetwattana
Apichaya Puangpetch
Santirhat Prommas
Suwannee Sirilerttrakul
Budsaba Rerkarmnuaychoke
Sansanee Wongwaisayawan
Chonlaphat Sukasem
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Health Professions;Medicine
Issue Date: 1-Jan-2018
Citation: Journal of Clinical Laboratory Analysis. Vol.32, No.1 (2018)
Abstract: © 2017 Wiley Periodicals, Inc. Background: Irinotecan (CPT-11) is chemotherapy used mainly in the metastatic colorectal cancer. The purpose of this study was to develop and validate the LC-MS/MS for the simultaneous determination of CPT-11, SN-38, and SN-38G. Methods: A 100 μL of plasma was prepared after protein precipitation and analyzed on a C18 column using 0.1% acetic acid in water and 0.1% acetic acid in acetonitrile as mobile phases. The mass spectrometer worked with multiple reaction monitoring (MRM) in positive scan mode. The standard curves were linear on a concentration range of 5-10 000 ng/mL for CPT-11, 5-1000 ng/mL for SN-38, and 8-1000 ng/mL for SN-38G. Results: In this assay, the intra and interday precision consisted of ≤9.11% and ≤11.29% for CPT-11, ≤8.70% and 8.31% for SN-38, and ≤9.90 and 9.64% for SN-38G. Conclusion: This method was successfully used to quantify CPT-11, SN-38, and SN-38G and applied to a pharmacokinetic study.
ISSN: 10982825
Appears in Collections:Scopus 2018

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