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Title: Chromatin immunoprecipitation sequencing (ChIP-seq) for transcription factors and chromatin factors in Arabidopsis thaliana roots: From material collection to data analysis
Authors: Sandra Cortijo
Varodom Charoensawan
François Roudier
Philip A. Wigge
Reproduction et Développement des Plantes
University of Cambridge
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jan-2018
Citation: Methods in Molecular Biology. Vol.1761, (2018), 231-248
Abstract: © 2018, Springer Science+Business Media, LLC. Chromatin immunoprecipitation combined with next-generation sequencing (ChIP-seq) is a powerful technique to investigate in vivo transcription factor (TF) binding to DNA, as well as chromatin marks. Here we provide a detailed protocol for all the key steps to perform ChIP-seq in Arabidopsis thaliana roots, also working on other A. thaliana tissues and in most non-ligneous plants. We detail all steps from material collection, fixation, chromatin preparation, immunoprecipitation, library preparation, and finally computational analysis based on a combination of publicly available tools.
ISSN: 10643745
Appears in Collections:Scopus 2018

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