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Title: Enrichment of human Vγ9Vδ2 T lymphocytes by magnetic poly(divinylbenzene-: Co -glycidyl methacrylate) colloidal particles conjugated with specific antibody
Authors: Piamsiri Sawaisorn
Tienrat Tangchaikeeree
Duangporn Polpanich
Panuwat Midoeng
Rachanee Udomsangpetch
Abdelhamid Elaissari
Kulachart Jangpatarapongsa
Laboratoire d'Automatique et de Génie des Procédés
Mahidol University
Phramongkutklao College of Medicine
Thailand National Science and Technology Development Agency
Keywords: Chemical Engineering;Chemistry
Issue Date: 1-Jan-2018
Citation: RSC Advances. Vol.8, No.26 (2018), 14393-14400
Abstract: © 2018 The Royal Society of Chemistry. γδ T cells play a significant role in protection against cancer. Purification of γδ T cells is needed for insight when studying their anti-cancer functionality and their utilization in adoptive cell therapy. To improve the purification of γδ T cells, in this work, a composite material based on magnetic nanoparticles was developed for purification of Vγ9Vδ2 T cells, the predominant subset of γδ T lymphocytes in human peripheral blood. The epoxy-functionalized magnetic poly(divinylbenzene-co-glycidyl methacrylate) particles (mPDGs) were bio-conjugated with anti-human Vδ2 antibody to provide specific recognition sites for T cell receptors of Vγ9Vδ2 T cells. Using fluorescence-activated cell sorting (FACS) analysis, separation of Vγ9Vδ2 T cells from peripheral blood mononuclear cells of healthy donors was confirmed with high purity [89.77% (range 87.00-91.80, n = 3)]. More interestingly, the immobilized particles did not affect the viability of purified cells as high cell viability was indicated (>90%). By combining the properties of magnetic nanoparticles with specific antibodies, these immobilized particles were shown to be used as a cell-friendly purification tool of Vγ9Vδ2 T lymphocytes without any limits for the further use of cells. The purified Vγ9Vδ2 T cells using the antibody-immobilized epoxy-functionalized mPDGs could be used directly without a detachment step for further cultivation and expansion. This highlights the advantages of this method in allowing the study of cell function and further investigation of such rare T cell populations in immunotherapy.
ISSN: 20462069
Appears in Collections:Scopus 2018

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