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Title: Determination of the marker diarylheptanoid phytoestrogens in Curcuma comosa rhizomes and selected herbal medicinal products by HPLC-DAD
Authors: Bancha Yingngam
Adelheid Brantner
Damrongsak Jinarat
Rawiwun Kaewamatawong
Wandee Rungseevijitprapa
Apichart Suksamrarn
Pawinee Piyachaturawat
Ratchanaporn Chokchaisiri
University of Phayao
Ubon Rajathanee University
Ramkhamhaeng University
Karl-Franzens-Universitat Graz
Mahidol University
Keywords: Chemistry;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jan-2018
Citation: Chemical and Pharmaceutical Bulletin. Vol.66, No.1 (2018), 65-70
Abstract: © 2018 The Pharmaceutical Society of Japan. A method for quantification of diarylheptanoids in Curcuma comosa rhizomes and selected pharmaceutical preparations was established by using HPLC-diode array detector (DAD). The chromatographic separation of three diarylheptanoids [(3S)-1-(3,4-dihydroxy-phenyl)-7-phenyl-(6E)-6-hepten-3-ol (1), (3R)-1,7- diphenyl-(4E,6E)-4,6-heptadien-3-ol (2), and (3S)-1,7-diphenyl-(6E)-6-hepten-3-ol (3)] was performed on a Luna C18 analytical column using gradient elution with 0.5% acetic acid in water and acetonitrile with a flow rate of 1 mL/min and a column temperature of 35°C. The calibration curves for the analytes showed good linearity (R 2 >0.999), high precision (relative standard deviation (RSD) <2%) and acceptable recovery (98.35-103.90%, RSD <2%). The limit of detection (LOD) and limit of quantification (LOQ) were 0.06-0.22 and 0.18-0.69 μg/mL, respectively. The results of all validated parameters were within the limits according to the International Conference on Harmonization (ICH) Guidelines. The established method was successfully applied for qualitative and quantitative determination of the three constituents in different samples of C. comosa and some commercial products in capsules. The simplicity, rapidity, and reliability of the method could be useful for the fingerprint analysis and standardization of diarylheptanoids, which are responsible for the estrogenic activity in raw materials and herbal medicinal products of C. comosa.
ISSN: 13475223
Appears in Collections:Scopus 2018

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