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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/45541
Title: Genomes of trombidid mites reveal novel predicted allergens and laterally transferred genes associated with secondary metabolism
Authors: Xiaofeng Dong
Kittipong Chaisiri
Dong Xia
Stuart D. Armstrong
Yongxiang Fang
Martin J. Donnelly
Tatsuhiko Kadowaki
John W. McGarry
Alistair C. Darby
Benjamin L. Makepeace
Xi'an Jiaotong-Liverpool University
Royal Veterinary College University of London
Liverpool School of Tropical Medicine
University of Liverpool
Mahidol University
Xuzhou Normal University
Keywords: Computer Science;Medicine
Issue Date: 1-Dec-2018
Citation: GigaScience. Vol.7, No.12 (2018)
Abstract: © 2018 The Author(s). Abstract Background Trombidid mites have a unique life cycle in which only the larval stage is ectoparasitic. In the superfamily Trombiculoidea ("chiggers"), the larvae feed preferentially on vertebrates, including humans. Species in the genus Leptotrombidium are vectors of a potentially fatal bacterial infection, scrub typhus, that affects 1 million people annually. Moreover, chiggers can cause pruritic dermatitis (trombiculiasis) in humans and domesticated animals. In the Trombidioidea (velvet mites), the larvae feed on other arthropods and are potential biological control agents for agricultural pests. Here, we present the first trombidid mites genomes, obtained both for a chigger, Leptotrombidium deliense, and for a velvet mite, Dinothrombium tinctorium. Results Sequencing was performed using Illumina technology. A 180 Mb draft assembly for D. tinctorium was generated from two paired-end and one mate-pair library using a single adult specimen. For L. deliense, a lower-coverage draft assembly (117 Mb) was obtained using pooled, engorged larvae with a single paired-end library. Remarkably, both genomes exhibited evidence of ancient lateral gene transfer from soil-derived bacteria or fungi. The transferred genes confer functions that are rare in animals, including terpene and carotenoid synthesis. Thirty-seven allergenic protein families were predicted in the L. deliense genome, of which nine were unique. Preliminary proteomic analyses identified several of these putative allergens in larvae. Conclusions Trombidid mite genomes appear to be more dynamic than those of other acariform mites. A priority for future research is to determine the biological function of terpene synthesis in this taxon and its potential for exploitation in disease control.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059794955&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/45541
ISSN: 2047217X
Appears in Collections:Scopus 2018

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