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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/45698
Title: Platelet-rich plasma stimulated proliferation, migration, and attachment of cultured periodontal ligament cells
Authors: Kanyawat Rattanasuwan
Supanee Rassameemasmaung
Sirichai Kiattavorncharoen
Anongporn Sirikulsathean
Jarinee Thorsuwan
Wilasinee Wongsankakorn
Mahidol University
Ranong Hospital
Private Practice
Keywords: Dentistry
Issue Date: 1-Oct-2018
Citation: European Journal of Dentistry. Vol.12, No.4 (2018), 469-474
Abstract: © 2018 European Journal of Dentistry | Published by Wolters Kluwer - Medknow. Objective: The aim of this study is to evaluate the effects of platelet-rich plasma (PRP) on the proliferation, migration, and attachment of cultured periodontal ligament (PDL) cells. Materials and Methods: 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to assess number of PDL cells cultured in medium with or without PRP. Cell migration toward medium with or without PRP was assessed using the Boyden chamber. Cell attachment was assessed by counting cells on PRP or non-PRP coated dentin specimens. Group differences were analyzed using two-way ANOVA at 0.05 significance level. Results: In the MTT and cell migration assay, the number of cells in 5% and 10% PRP-treated groups were significantly higher than that in the non-PRP-treated group (P < 0.05). In the attachment assay, the number of cells on the dentin specimens in 10% PRP-treated group was significantly higher than that in the non-PRP treated group (P < 0.05). Conclusion: PRP could stimulate proliferation, migration, and attachment of PDL cells.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85054505169&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/45698
ISSN: 13057464
13057456
Appears in Collections:Scopus 2018

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