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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/45968
Title: Small plaque size variant of chikungunya primary isolate showed reduced virulence in mice
Authors: Thitigun Jaimipak
Sutee Yoksan
Sukathida Ubol
Rojjanaporn Pulmanausahakul
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Sep-2018
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.36, No.3 (2018), 201-205
Abstract: © 2018, Allergy and Immunology Society of Thailand. All rights reserved. Background: Plaque size is a common feature of viral characterization. Small plaque size is used as a marker of attenuation for live-attenuated vaccine development. Objective: To investigate whether the naturally occurring plaque size variation reflects virulence of the variants of chikungunya virus (CHIKV). Methods: We selected and purified a variant with small plaque size from the primary isolate. The viral variant was tested for the plaque morphology, in vitro growth kinetics and mouse neurovirulence in comparison with the parental wild type. Results: The small plaque size variant showed stable homogenous small plaques after 4 plaque purifications. The small plaque virus grew slower and to the lower titer when compared with wild type virus. After 21 days of infection, mice that received small plaque virus showed 98% survival rate while 74% of mice survived after infected with wild type virus. Conclusion: The small plaque size variant of CHIKV can be obtained by plaque purification and the virus displays decreased virulence.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85054449439&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/45968
ISSN: 22288694
0125877X
Appears in Collections:Scopus 2018

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