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Title: Deciphering critical amino acid residues to modify and enhance the binding affinity of ankyrin scaffold specific to capsid protein of human immunodeficiency virus type 1
Authors: Somphot Saoin
Tanchanok Wisitponchai
Kannaporn Intachai
Koollawat Chupradit
Sutpirat Moonmuang
Sawitree Nangola
Kuntida Kitidee
Kanda Fanhchaksai
Vannajan Sanghiran Lee
Saw See Hong
Pierre Boulanger
Phimonphan Chuankhayan
Chun Jung Chen
Chatchai Tayapiwatana
University of Phayao
University of Malaya
Université Claude Bernard Lyon 1
National Synchrotron Radiation Research Center Taiwan
National Tsing Hua University
National Cheng Kung University
Mahidol University
Chiang Mai University
Commission on Higher Education
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jun-2018
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.36, No.2 (2018), 126-135
Abstract: © 2018, Allergy and Immunology Society of Thailand. All rights reserved. Background: Ank GAG 1D4 is an artificial ankyrin repeat protein which recognizes the capsid protein (CA) of the human immunodeficiency virus type 1 (HIV-1) and exhibits the intracellular antiviral activity on the viral assembly process. Improving the binding affinity of Ank GAG 1D4 would potentially enhance the Ank GAG 1D4-mediated antiviral activity. Objective: To augment the affinity of Ank GAG 1D4 scaffold towards its CA target, through computational predictions and experimental designs. Method: Three dimensional structure of the binary complex formed by Ank GAG 1D4 docked to the CA was used as a model for van der Waals (vdW) binding energy calculation. The results generated a simple guideline to select the amino acids for modifications. Following the predictions, modified Ank GAG 1D4 proteins were produced and further evaluated for their CA-binding activity, using ELISA-modified method and bio-layer interferometry (BLI). Results: Tyrosine at position 56 (Y56) in Ank GAG 1D4 was experimentally identified as the most critical residue for CA binding. Rational substitutions of this residue diminished the binding affinity. However, vdW calculation preconized to substitute serine for tyrosine at position 45. Remarkably, the affinity for the viral CA was significantly enhanced in Ank GAG 1D4-S45Y mutant, with no alteration of the target specificity. Conclusions: The S-to-Y mutation at position 45, based on the prediction of interacting amino acids and on vdW binding energy calculation, resulted in a significant enhancement of the affinity of Ank GAG 1D4 ankyrin for its CA target. Ank GAG 1D4-S45Y mutant represented the starting point for further construction of variants with even higher affinity towards the viral CA, and higher therapeutic potential in the future.
ISSN: 22288694
Appears in Collections:Scopus 2018

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