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Title: Quantification of fel d 1 in house dust samples of cat allergic patients by using monoclonal antibody specific to a novel IgE-binding epitope
Authors: Natt Tasaniyananda
Anchalee Tungtrongchitr
Watee Seesuay
Yuwaporn Sakolvaree
Pisinee Aiumurai
Nitaya Indrawattana
Wanpen Chaicumpa
Nitat Sookrung
Mahidol University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Mar-2018
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.36, No.1 (2018), 8-15
Abstract: © 2018, Allergy and Immunology Society of Thailand. All rights reserved. Background: Avoidance of allergen exposure is an effective measure for preventing naïve and allergic individuals from sensitization (primary intervention) and disease aggravation (secondary intervention), respectively. Regular monitoring of the allergens in the environment is required for the effective intervention. Thus, there is a need for cost-effective test kits for environmental allergen quantifications. Objective: To invent a test kit for quantification of cat major allergen, Fel d 1. Methods: A mouse monoclonal antibody (MAb) specific to the newly identified IgE-binding conformational epitope of the cat major allergen (Fel d 1) and rabbit polyclonal IgG to recombinant Fel d 1 were used as allergen capture and detection reagents, respectively. Native Fel d 1 was used in constructing a standard curve. Results and Conclusion: Sixteen of 36 dust samples collected from houses of cat allergic subjects in Bangkok contained Fel d 1 above 0.29 µg/gram of dust which is considered as a novel threshold level for causing cat allergy sensitization or symptoms. Among them, 7 samples contained the allergen exceeding 2.35 µg/gram of dust which is the level that would aggravate asthma. Results of the allergen quantification using the locally made test kit showed strong correlation (r = 0.923) with the allergen quantification using commercialized reagents. The assay using MAb to Fel d 1 IgE-binding epitope of this study has potential application as an economic and practical tool for cat allergy intervention measure especially in localities where health resources are relatively limited.
ISSN: 22288694
Appears in Collections:Scopus 2018

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