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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/46137
Title: Assessment of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification and biotyping of the pathogenic oomycete Pythium insidiosum
Authors: Theerapong Krajaejun
Tassanee Lohnoo
Paisan Jittorntam
Atthapol Srimongkol
Yothin Kumsang
Wanta Yingyong
Thidarat Rujirawat
Onrapak Reamtong
Suthee Mangmee
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Mahidol University
Keywords: Medicine
Issue Date: 1-Dec-2018
Citation: International Journal of Infectious Diseases. Vol.77, (2018), 61-67
Abstract: © 2018 The Author(s) Objective: Pythiosis is a life-threatening infectious disease caused by the oomycete Pythium insidiosum. The disease has been increasingly reported worldwide. Most patients with pythiosis undergo surgical removal of an infected organ. Early diagnosis contributes to better prognosis of pythiosis patients. Here, we assessed the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for identification and biotyping of P. insidiosum. Methods: A comprehensive set of mass spectra of P. insidiosum was generated to evaluate MALDI-TOF MS for identification and biotyping of P. insidiosum. Results: MALDI-TOF MS accurately identified all 13 P. insidiosum strains tested, at the species level. Mass spectra of P. insidiosum did not match any other microorganisms, including fungi (i.e., Aspergillus species, Fusarium species, and fungal species of the class Zygomycetes), which have similar microscopic morphologies with this oomycete. MALDI-TOF MS- and rDNA sequence-based biotyping methods consistently classified P. insidiosum into three groups: Clade-I (American strains), II (Asian and Australian strains), and III (mostly Thai strains). Conclusions: MALDI-TOF MS has been successfully used for identification and biotyping of P. insidiosum. The obtained mass spectral database allows clinical microbiology laboratories, well-equipped with a MALDI-TOF mass spectrometer, to conveniently identify P. insidiosum, without requiring any pathogen-specific reagents (i.e., antigen, antibody or primers).
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85055732208&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/46137
ISSN: 18783511
12019712
Appears in Collections:Scopus 2018

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