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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/46466
Title: Synthetic curcuminoid analogues abrogate oxidation-induced cell death and promote myogenic differentiation of C2C12 mouse myoblasts
Authors: Chittipong Tipbunjong
Piyawat Sookbangnop
Vachiraporn Ajavakom
Apichart Suksamrarn
Yindee Kitiyanant
Chumpol Pholpramool
Ramkhamhaeng University
Mahidol University
Prince of Songkla University
Keywords: Medicine;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Aug-2018
Citation: Tropical Journal of Pharmaceutical Research. Vol.17, No.8 (2018), 1483-1489
Abstract: © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria and 2018 The authors. Purpose: To investigate the ability of two synthetic curcuminoid analogues, 6-(4-hydroxy-3-methoxyphenethyl)-5-(3-(4-hydroxy-3-methoxyphenyl)propanoyl)-4-phenyl-3,4-dihydropyrimidin-2(1H)-one (compound A) and 6-(4-hydroxy-3-methoxyphenethyl)-4-(4-hydroxy-3-methoxyphenyl)-5-(3-(4-hydroxy-3-methoxyphenyl)propanoyl)-3,4-dihydropyrimidin-2(1H)-one (compound B), to protect against oxidation-induced cell death and the potential to enhance proliferation and differentiation of C2C12 myoblast cells. Methods: Antioxidant activity of curcuminoid analogues was evaluated by DPPH assay. The cytotoxic activity of the compounds (0-25 mM) on C2C12 myoblasts was determined by MTT assay while the effect on cell proliferation was assessed by BrdU uptake. Myoblast cell differentiation was measured by the formation of myotubes and myosin heavy chain (MHC) protein expression using immunofluorescence staining and Western blotting, respectively. Results: Both curcuminoid analogues exhibited strong anti-oxidant activity of up to 3-fold greater than that of ascorbic acid, and were non-toxic to C2C12 myoblasts at concentrations up to 25 mM. Furthermore, these curcuminoid analogues mitigated myoblast cell death induced by oxidative stress. Notably, both analogues (10 nM) had no effect on cell proliferation. However, only compound A significantly enhanced myoblast differentiation comparable to the effects of dihydrotestosterone (1 µM) and estradiol (10 nM). Conclusion: The results suggest that compound A may serve as a lead compound for the development of suitable therapeutic agents for muscle injuries and diseases.
URI: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85052877718&origin=inward
http://repository.li.mahidol.ac.th/dspace/handle/123456789/46466
ISSN: 15969827
15965996
Appears in Collections:Scopus 2018

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