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Title: A rapid immunochromatography test based on Hcp1 is a potential point-of-care test for serological diagnosis of melioidosis
Authors: Phornpun Phokrai
Wisansanee Karoonboonyanan
Nida Thanapattarapairoj
Chidchanok Promkong
Adul Dulsuk
Sirikamon Koosakulnirand
Sasha Canovali
Nitaya Indrawattana
Yaowaruk Jutrakul
Vanaporn Wuthiekanun
Direk Limmathurotsakul
Paul J. Brett
Mary N. Burtnick
Ganjana Lertmemongkolchai
Narisara Chantratita
Udon Thani Center Hospital
Khon Kaen University
Khon Kaen Regional Hospital
Mahidol University
University of Hawaii at Manoa John A. Burns School of Medicine
University of Nevada School of Medicine
Nakhon Phanom Hospital
Keywords: Medicine
Issue Date: 1-Aug-2018
Citation: Journal of Clinical Microbiology. Vol.56, No.8 (2018)
Abstract: © 2018 Phokrai et al. Melioidosis is a fatal infectious disease caused by the environmental bacterium Burkholderia pseudomallei. It is highly endemic in Asia and northern Australia but neglected in many other tropical countries. Melioidosis patients have a wide range of clinical manifestations, and definitive diagnosis requires bacterial culture, which can be time-consuming. A reliable rapid serological tool is greatly needed for disease surveillance and diagnosis. We previously demonstrated by enzyme-linked immunosorbent assay (ELISA) that a hemolysin-coregulated protein (Hcp1) is a promising target for serodiagnosis of melioidosis. In this study, we developed a rapid immunochromatography test (ICT) using Hcp1 as the target antigen (Hcp1-ICT). We evaluated this test for specific antibody detection using serum samples obtained from 4 groups of human subjects, including the following: (i) 487 culture-confirmed melioidosis patients from four hospitals in northeast Thailand; (ii) 202 healthy donors from northeast Thailand; (iii) 90 U.S. healthy donors; and (iv) 207 patients infected with other organisms. Compared to culture results as a gold standard, the sensitivity of ICT for all hospitals was 88.3%. The specificities for Thai donors and U.S. donors were 86.1% and 100%, respectively, and the specificity for other infections was 91.8%. The results of the Hcp1-ICT demonstrated 92.4% agreement with the Hcp1-ELISA results with a kappa value of 0.829, indicating that the method is much improved compared with the current serological method, the indirect hemagglutination assay (IHA) (69.5% sensitivity and 67.6% specificity for Thais). The Hcp1-ICT represents a potential point-of-care (POC) test and may be used to replace the IHA for screening of melioidosis in hospitals as well as in resource-limited areas.
ISSN: 1098660X
Appears in Collections:Scopus 2018

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