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Title: Evaluation of a rapid diagnostic test for detection of burkholderia pseudomallei in the Lao people's democratic republic
Authors: Kate L. Woods
Latsaniphone Boutthasavong
Caoimhe NicFhogartaigh
Sue J. Lee
Viengmon Davong
David P. AuCoin
David A.B. Dancea
London School of Hygiene & Tropical Medicine
University of Oxford
Mahidol University
Royal London Hospital
University of Nevada School of Medicine
Mahosot Hospital
Keywords: Medicine
Issue Date: 1-Jul-2018
Citation: Journal of Clinical Microbiology. Vol.56, No.7 (2018)
Abstract: Copyright © 2018 Woods et al. Burkholderia pseudomallei causes significant global morbidity and mortality, with the highest disease burden in parts of Asia where culture-based diagnosis is often not available. We prospectively evaluated the Active Melioidosis Detect (AMD; InBios International, USA) lateral flow immunoassay (LFI) for rapid detection of B. Pseudomallei in turbid blood cultures, pus, sputum, sterile fluid, urine, and sera. The performance of this test was compared to that of B. Pseudomallei detection using monoclonal antibody latex agglutination (LA) and immunofluorescence assays (IFA), with culture as the gold standard. AMD was 99% (99/100; 95% confidence interval, 94.6 to 100%) sensitive and 100% (308/308; 98.8 to 100%) specific on turbid blood culture bottles, with no difference from LA or IFA. AMD specificity was 100% on pus (122/122; 97.0 to 100%), sputum (20/20; 83.2 to 100%), and sterile fluid (44/44; 92 to 100%). Sensitivity on these samples was as follows: pus, 47.1% (8/17; 23.0 to 72.2%); sputum, 33.3% (1/3; 0.84 to 90.6%); and sterile fluid, 0% (0/2; 0 to 84.2%). For urine samples, AMD had a positive predictive value of 94% (32/34; 79.7 to 98.5%) for diagnosing melioidosis in our cohort. AMD sensitivity on stored sera, collected prospectively from melioidosis cases during this study, was 13.9% (5/36; 4.7% to 29.5%) compared to blood culture samples taken on the same day. In conclusion, AMD is an excellent tool for rapid diagnosis of melioidosis from turbid blood cultures and maintains specificity across all sample types. It is a promising tool for urinary antigen detection, which could revolutionize diagnosis of melioidosis in resource-limited settings. Further work is required to improve sensitivity on nonblood culture samples.
ISSN: 1098660X
Appears in Collections:Scopus 2018

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