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Title: HBA as a potential alternative to the tunel assay for assessment of dna integrity in asthenozoospermic spermatozoa
Authors: Pitak Laokirkkiat
Paweena Sarakarnkosol
Isarin Thanaboonyawat
Somsin Petyim
Japarath Prechapanich
Suphadtra Phornwilardsiri
Noira Nigmatova
Roungsin Choavaratana
Taksin Hospital
Faculty of Medicine, Siriraj Hospital, Mahidol University
Genome Astana
Keywords: Medicine
Issue Date: 1-Jan-2018
Citation: Siriraj Medical Journal. Vol.70, No.1 (2018), 70-76
Abstract: © 2018, Faculty of Medicine Siriraj Hospital, Mahidol University. Objective: This study was conducted to evaluate the appropriateness of the hyaluronan binding assay (HBA) for assessment of DNA integrity indirectly in asthenozoospermic spermatozoa, by studying its correlation with the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. Methods: Thirty asthenozoospermic semen samples of infertile couples were recruited. Semen analysis was performed before being processed by HBA and the TUNEL assay. All the semen parameters were recorded and analyzed. Results: The mean age of the patients was 38.1 ± 6.4 years. The average total and progressive motility was 33.5 ± 11.0% and 21.9 ± 6.6%, respectively. The mean TUNEL-positive and hyaluronan-bound sperm were 17.8 ± 6.4% and 57.2 ± 14.5%, respectively. Pearson correlation coefficient showed a high negative correlation between HBA and the TUNEL assay (r = -0.73, p < 0.001). The intraclass correlation coefficients revealed a high level of agreement between two observers who evaluated the TUNEL-positive and hyaluronan-bound sperm [0.81 (95% CI: 0.65-0.91) and 0.92 (95% CI: 0.84-0.96)], respectively. Conclusion: The study results showed a high negative correlation between HBA and the TUNEL assay. This suggests that HBA may be used as a viable intact DNA spermatozoa identification method, as a potential alternative to the TUNEL assay, in asthenozoospermia sperm.
ISSN: 22288082
Appears in Collections:Scopus 2018

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