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dc.contributor.authorSuporn Paksanonten_US
dc.contributor.authorKitisak Sintiprungraten_US
dc.contributor.authorThatcha Yimthinen_US
dc.contributor.authorPornpan Pumiraten_US
dc.contributor.authorSharon J. Peacocken_US
dc.contributor.authorNarisara Chantratitaen_US
dc.contributor.otherLondon School of Hygiene & Tropical Medicineen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2019-08-28T07:10:57Z-
dc.date.available2019-08-28T07:10:57Z-
dc.date.issued2018-12-01en_US
dc.identifier.citationScientific Reports. Vol.8, No.1 (2018)en_US
dc.identifier.issn20452322en_US
dc.identifier.other2-s2.0-85048713414en_US
dc.identifier.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048713414&origin=inwarden_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/47470-
dc.description.abstract© 2018 The Author(s). Burkholderia pseudomallei is a flagellated, gram-negative environmental bacterium that causes melioidosis, a severe infectious disease of humans and animals in tropical areas. We hypothesised that B. pseudomallei may undergo phenotypic adaptation in response to an increase in growth temperature. We analysed the growth curves of B. pseudomallei strain 153 cultured in Luria-Bertani broth at five different temperatures (25 °C-42 °C) and compared the proteomes of bacteria cultured at 37 °C and 42 °C. B. pseudomallei exhibited the highest growth rate at 37 °C with modest reductions at 30 °C, 40 °C and 42 °C but a more marked delay at 25 °C. Proteome analysis revealed 34 differentially expressed protein spots between bacterial cultures at 42 °C versus 37 °C. These were identified as chaperones (7 spots), metabolic enzymes (12 spots), antioxidants (10 spots), motility proteins (2 spots), structural proteins (2 spots) and hypothetical proteins (1 spot). Of the 22 down-regulated proteins at 42 °C, redundancy in motility and antioxidant proteins was observed. qRT-PCR confirmed decreased expression of fliC and katE. Experiments on three B. pseudomallei strains demonstrated that these had the highest motility, greatest resistance to H2O2 and greatest tolerance to salt stress at 37 °C. Our data suggest that temperature affects B. pseudomallei motility and resistance to stress.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048713414&origin=inwarden_US
dc.subjectMultidisciplinaryen_US
dc.titleEffect of temperature on Burkholderia pseudomallei growth, proteomic changes, motility and resistance to stress environmentsen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1038/s41598-018-27356-7en_US
Appears in Collections:Scopus 2018

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