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Title: Prime-boost vaccination with recombinant protein and adenovirus-vector expressing Plasmodium vivax circumsporozoite protein (CSP) partially protects mice against Pb/Pv sporozoite challenge
Authors: Tarsila Mendes De Camargo
Elisângela Oliveira De Freitas
Alba Marina Gimenez
Luciana Chagas Lima
Karina De Almeida Caramico
Kátia Sanches Françoso
Oscar Bruna-Romero
Chiara Andolina
François Nosten
Laurent Rénia
Hildegund C.J. Ertl
Ruth S. Nussenzweig
Victor Nussenzweig
Mauricio M. Rodrigues
Arturo Reyes-Sandoval
Irene S. Soares
A-Star, Singapore Immunology Network
NYU School of Medicine
Universidade Federal de Santa Catarina
Universidade Federal de Sao Paulo
Mahidol University
The Wistar Institute
Nuffield Department of Clinical Medicine
Universidade de Sao Paulo - USP
Keywords: Multidisciplinary
Issue Date: 1-Dec-2018
Citation: Scientific Reports. Vol.8, No.1 (2018)
Abstract: © 2018 The Author(s). Vaccine development against Plasmodium vivax malaria lags behind that for Plasmodium falciparum. To narrow this gap, we administered recombinant antigens based on P. vivax circumsporozoite protein (CSP) to mice. We expressed in Pichia pastoris two chimeric proteins by merging the three central repeat regions of different CSP alleles (VK210, VK247, and P. vivax-like). The first construct (yPvCSP-AllFL) contained the fused repeat regions flanked by N- and C-terminal regions. The second construct (yPvCSP-AllCT) contained the fused repeat regions and the C-terminal domain, plus RI region. Mice were vaccinated with three doses of yPvCSP in adjuvants Poly (I:C) or Montanide ISA720. We also used replication-defective adenovirus vectors expressing CSP of human serotype 5 (AdHu5) and chimpanzee serotype 68 (AdC68) for priming mice which were subsequently boosted twice with yPvCSP proteins in Poly (I:C) adjuvant. Regardless of the regime used, immunized mice generated high IgG titres specific to all CSP alleles. After challenge with P. berghei ANKA transgenic parasites expressing Pb/PvVK210 or Pb/PvVK247 sporozoites, significant time delays for parasitemia were observed in all vaccinated mice. These vaccine formulations should be clinically tried for their potential as protective universal vaccine against P. vivax malaria.
ISSN: 20452322
Appears in Collections:Scopus 2018

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