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Title: Characterization of TRH/GnRH-like peptides in the sea cucumber, Holothuria scabra, and their effects on oocyte maturation
Authors: Arada Chaiyamoon
Ruchanok Tinikul
Nittiya Nontunha
Supakant Chaichotranunt
Tanes Poomtong
Prasert Sobhon
Yotsawan Tinikul
Khon Kaen University
Mahidol University
Royal Fisheries Initiated Projects and Special Activities Division
Coastal Fisheries Research and Development Center
Keywords: Agricultural and Biological Sciences
Issue Date: 15-Mar-2020
Citation: Aquaculture. Vol.518, (2020)
Abstract: © 2019 Elsevier B.V. Thyrotropin/Gonadotropin-releasing hormone (TRH/GnRH) is a neurohormone that plays important roles in regulation of several physiological processes of invertebrate species, including echinoderms. The aims of this study were to characterize for the first time a TRH/GnRH-like peptide in the sea cucumber, Holothuria scabra. We have isolated a full-length cDNA for a TRH/GnRH preprohormone gene from the radial nerve cord of H. scabra. We found that H. scabra (hs)TRH/GnRH precursor comprised two types of peptides, namely, the short TRH/GnRH-like peptides and the long TRH/GnRH-like peptides. The expression of hsTRH/GnRH gene, was intense in radial nerve cord, nerve ring and mature ovary by using RT-PCR. By in situ hybridization the transcripts of hsTRH/GnRH was detected at a high level within the late stages (oocyte stages 3 and 4). In the functional assays, injections of the short TRH/GnRH-like peptides could significantly accelerate gonadal development as reflected by increased gonadal-somatic index (GSI), and stimulated gametogenesis, as reflected by significantly increased oocyte proliferation. Taken together, our new discovery of the TRH/GnRH-like peptides in H. scabra contributes important new insights into the evolution of TRH/GnRH neuropeptide system in this sea cucumber species, and these neuropeptides could be potentially applied in the sea cucumber aquaculture.
ISSN: 00448486
Appears in Collections:Scopus 2020

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