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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/49519
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dc.contributor.authorManawat Suwannarojen_US
dc.contributor.authorThatcha Yimthinen_US
dc.contributor.authorChamaiporn Fukruksaen_US
dc.contributor.authorParamaporn Muangpaten_US
dc.contributor.authorTemsiri Yooyangketen_US
dc.contributor.authorSarunporn Tandhavananten_US
dc.contributor.authorAunchalee Thanwisaien_US
dc.contributor.authorApichat Vittaen_US
dc.contributor.otherNaresuan Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2020-01-27T03:28:20Z-
dc.date.available2020-01-27T03:28:20Z-
dc.date.issued2020-01-01en_US
dc.identifier.citationJournal of Applied Entomology. (2020)en_US
dc.identifier.issn14390418en_US
dc.identifier.issn09312048en_US
dc.identifier.other2-s2.0-85077874411en_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/49519-
dc.description.abstract© 2020 Blackwell Verlag GmbH Aedes aegypti is an insect vector that transmits several viruses affecting humans worldwide. Entomopathogenic nematodes (EPNs) and their symbiotic bacteria are organisms with the potential to control many insects. In this study, we did a survey aimed to identify EPNs and their symbiotic bacteria and evaluate the larvicidal activity of bacteria against Ae. aegypti. We collected 540 soil samples from 108 sites in Phitsanulok Province, lower northern Thailand. Baiting techniques and White traps were used to isolate EPNs from soil samples. By sequencing of 28S rDNA and internal transcribed spacer regions, 51 EPN isolates were identified as Steinernema surkhetense (35 isolates), Heterorhabditis indica (14 isolates) and Heterorhabditis sp. SGmg3 (two isolates). Based on sequencing of a partial region of the recA gene, 35 isolates of Xenorhabdus were identified as Xenorhabdus stockiae, and 20 Photorhabdus isolates were identified as Photorhabdus luminescens subsp. akhurstii (10 isolates), P. luminescens subsp. hainanensis (seven isolates) and P. asymbiotica subsp. australis (three isolates). Screening for larvicidal activity of bacteria against Ae. aegypti was performed in the laboratory. Xenorhabdus WB5.4 and Xenorhabdus WB12.5, which were closely related to X. stockiae, resulted in high mortality of Ae. aegypti (99.99% and 70%, respectively) at 96 hr after exposure. Comparing with control groups, mortality of Ae. aegypti larvae was low (1.11%–6.67%) after exposure for 24–96 hr. Our findings showed the potential of X. stockiae for controlling Ae. aegypti. Further studies are needed to elucidate the mechanisms through which these bacteria kill Ae. aegypti larvae.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85077874411&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.titleSurvey of entomopathogenic nematodes and associate bacteria in Thailand and their potential to control Aedes aegyptien_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1111/jen.12726en_US
dc.identifier.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85077874411&origin=inwarden_US
Appears in Collections:Scopus 2020

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