Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: Estimating Human Exposure to Rat Lungworm (Angiostrongylus cantonensis) on Hawai'i Island: A Pilot Study
Authors: Susan I. Jarvi
Praphathip Eamsobhana
Stefano Quarta
Kathleen Howe
Steven Jacquier
Alexandra Hanlon
Kirsten Snook
Robert McHugh
Zachariah Tman
Jill Miyamura
Kuilei Kramer
McKayla Meyer
University of Hawaii System
Virginia Polytechnic Institute and State University
University of California, Berkeley
Faculty of Medicine, Siriraj Hospital, Mahidol University
Hawai'i Health Information Corporation
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2020
Citation: The American journal of tropical medicine and hygiene. Vol.102, No.1 (2020), 69-77
Abstract: Angiostrongylus cantonensis is a zoonotic, parasitic nematode causing angiostrongyliasis or rat lungworm disease. Clinical diagnosis in humans is currently confirmed by detection of parasite DNA in cerebrospinal fluid. This study estimated human exposure to A. cantonensis in volunteer participants solicitated via public venues on east Hawai'i Island using blood-based tests. Antibodies were screened in sera by crude antigen ELISA, followed by a 31-kDa dot-blot test developed and validated in Thailand. Human participants (n = 435) donated blood samples and completed a questionnaire to self-report relevant symptomology or clinical diagnosis. Among symptoms reported by participants diagnosed by licensed clinicians, headaches, high eosinophil counts, stiff neck, fatigue, and joint pain were most severe during the initial 3 months of infection. ELISA results revealed 22% of the serum samples as positive, 46% as equivocal, and 32% as negative. A subset of 186 samples was tested by dot blot, with 30% testing positive and 70% testing negative. A significantly higher mean ELISA value was found among recently (2014-2015) clinically diagnosed participants as than among those with a diagnosis before 2010 (P = 0.027). All dot-blot positives were also ELISA positive and were significantly associated with higher ELISA values compared with dot-blot negatives (P = 0.0001). These results suggest that an ELISA using crude antigen isolated from adult A. cantonensis from Hawai'i may be an effective initial screening method for estimating exposure to A. cantonensis in Hawai'i and likewise suggest that dot-blot tests using the 31-kDa antigen exhibit efficacy as a diagnostic for exposure.
ISSN: 14761645
Appears in Collections:Scopus 2020

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.