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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/49809
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dc.contributor.authorMunazza Naziren_US
dc.contributor.authorDuangjai Tungmunnithumen_US
dc.contributor.authorShankhamala Boseen_US
dc.contributor.authorSamantha Droueten_US
dc.contributor.authorLaurine Garrosen_US
dc.contributor.authorNathalie Giglioli-Guivarc'hen_US
dc.contributor.authorBilal Haider Abbasien_US
dc.contributor.authorChristophe Hanoen_US
dc.contributor.otherQuaid-i-Azam Universityen_US
dc.contributor.otherUniversity of Azad Jammu and Kashmiren_US
dc.contributor.otherUniversité de Toursen_US
dc.contributor.otherUniversite d'Orleansen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherCNRS GDR3711en_US
dc.date.accessioned2020-01-27T07:25:44Z-
dc.date.available2020-01-27T07:25:44Z-
dc.date.issued2019-02-20en_US
dc.identifier.citationJournal of Agricultural and Food Chemistry. Vol.67, No.7 (2019), 1847-1859en_US
dc.identifier.issn15205118en_US
dc.identifier.issn00218561en_US
dc.identifier.other2-s2.0-85061484118en_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/49809-
dc.description.abstract© 2019 American Chemical Society. Ocimum basilicum L. (Purple basil) is a source of biologically active antioxidant compounds, particularly phenolic acids and anthocyanins. In this study, we have developed a valuable protocol for the establishment of in vitro callus cultures of O. basilicum and culture conditions for the enhanced production of distinct classes of phenylpropanoid metabolites such as hydroxycinnamic acid derivatives (caffeic acid, chicoric acid, rosmarinic acid) and anthocyanins (cyanidin and peonidin). Callus cultures were established by culturing leaf explants on Murashige and Skoog medium augmented with different concentrations of plant growth regulators (PGRs) [thidiazuron (TDZ), α-naphthalene acetic acid (NAA), and 6-benzyl amino purine (BAP)] either alone or in combination with 1.0 mg/L NAA. Among all the above-mentioned PGRs, NAA at 2.5 mg/L led to the highest biomass accumulation (23.2 g/L DW), along with total phenolic (TPP; 210.7 mg/L) and flavonoid (TFP; 196.4 mg/L) production, respectively. HPLC analysis confirmed the differential accumulation of phenolic acid [caffeic acid (44.67 mg/g DW), rosmarinic acid (52.22 mg/g DW), and chicoric acid (43.89 mg/g DW)] and anthocyanins [cyanidin (16.39 mg/g DW) and peonidin (10.77 mg/g DW)] as a function of the PGRs treatment. The highest in vitro antioxidant activity was determined with the ORAC assay as compared to the FRAP assay, suggesting the prominence of the HAT over the ET-based mechanism for the antioxidant action of callus extracts. Furthermore, in vivo results illustrated the protective action of the callus extract to limit the deleterious effects of UV-induced oxidative stress, ROS/RNS production, and membrane integrity in yeast cell culture. Altogether, these results clearly demonstrated the great potential of in vitro callus of O. basilicum as a source of human health-promoting antioxidant phytochemicals.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061484118&origin=inwarden_US
dc.subjectAgricultural and Biological Sciencesen_US
dc.subjectChemistryen_US
dc.titleDifferential Production of Phenylpropanoid Metabolites in Callus Cultures of Ocimum basilicum L. With Distinct in Vitro Antioxidant Activities and in Vivo Protective Effects against UV stressen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1021/acs.jafc.8b05647en_US
dc.identifier.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061484118&origin=inwarden_US
Appears in Collections:Scopus 2019

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