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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/49822
Title: Testing of a pond-side molecular diagnostic tool for the detection of white spot syndrome virus in shrimp aquaculture
Authors: Diana Minardi
Kelly S. Bateman
Agnieszka Kuzdzal
Michelle Stone
Justin Avant
Richard Condliffe
Paul Brotherton
Mark Laverick
Kallaya Sritunyalucksana
Ornchuma Itsathitphaisarn
Puttharat Baoprasertkul
Grant D. Stentiford
genedrive plc
Centre for the Environment Fisheries and Aquaculture Science
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Aquatic Animal Health Research and Development Division (AAHRDD)
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Feb-2019
Citation: Journal of the World Aquaculture Society. Vol.50, No.1 (2019), 18-33
Abstract: © 2018 Crown copyright. Journal of the Word Aquaculture Society © 2018 World Aquaculture Society White spot disease in penaeid shrimp is caused by the white spot syndrome virus (WSSV). It is the most economically important disease of farmed warm-water shrimp, causing extensive economic losses estimated from $8 to $15 billion since its emergence in the 1990s. Early diagnosis of disease is critical in the management of outbreaks and to avoid crop losses. Diagnosis of white spot disease is generally carried out in centralized laboratory settings using molecular biology approaches. However, this mode of testing can be expensive and time consuming, requiring laboratory equipment, highly trained laboratory personnel, dedicated laboratory space, and long-distance transportation of samples from field to lab. In-field diagnostics are gaining credence as tools for rapid and early animal disease detection, allowing diagnosticians and farmers to potentially manage disease outbreaks from the pond side. In the present study, we describe the development and application of a new in-field point-of-need diagnostic test and platform for the diagnosis of WSSV in remote settings (shrimp farms). We report its performance in laboratory and field settings and compare it with current gold-standard diagnostic approaches. We discuss the potential benefits (and barriers to uptake) of applying such testing in the global shrimp farming sector.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/49822
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85053916686&origin=inward
ISSN: 17497345
08938849
Appears in Collections:Scopus 2019

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