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Title: Cryopreservation of Arundina graminifolia (D. Don) hochr. Seeds using D cryo-plate method
Authors: K. Thammasiri
S. Prasongsom
P. Kongsawadworakul
N. Chuenboonngarm
T. Jenjittikul
P. Soonthornchainaksaeng
U. Viboonjun
A. Muangkroot
Mahidol University
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jan-2019
Citation: Acta Horticulturae. Vol.1234, (2019), 301-308
Abstract: © 2019 International Society for Horticultural Science. All Rights Reserved. In this study, cryopreservation of Arundina graminifolia seeds by D cryo-plate and encapsulation dehydration technique were investigated. The results showed that the D cryo-plate method gave the highest regrowth of 82%, followed by encapsulation-dehydration (74%). The D cryo-plate protocol is as follows: pour the alginate solution containing 2% (w/v) sodium alginate in calcium-free ½ MS basal medium with 0.4 M sucrose in the wells of the cryo-plate. For encapsulation-dehydration the protocol is the same as D cryo-plate without using cryo-plate. Place 20 to 50 seeds from 3-month-old selfing capsules in each well. Pour calcium chloride solution containing 0.1 M calcium chloride in ½ MS basal medium with 0.4 M sucrose. Expose to 0.4 M sucrose + 2 M glycerol (loading solution) for 30 min and then dehydrate under a laminar airflow cabinet for 3 h. Put each cryo-plate in a 2-mL cryotube and plunge into liquid nitrogen for 1 d. Warm in 1.2 M sucrose solution (unloading solution) for 15 min then remove the solution and culture cryopreserved beads on ½ MS agar medium.
ISSN: 24066168
Appears in Collections:Scopus 2019

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