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Title: Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia
Authors: Saengchan Senapin
Ha Thanh Dong
Watcharachai Meemetta
Warachin Gangnonngiw
Pakakul Sangsuriya
Rapeepun Vanichviriyakit
Molruedee Sonthi
Bunlung Nuangsaeng
Suan Sunandha Rajabhat University
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Burapha University
Thailand National Science and Technology Development Agency
Keywords: Agricultural and Biological Sciences
Issue Date: 1-Jan-2019
Citation: Journal of Fish Diseases. Vol.42, No.1 (2019), 119-127
Abstract: © 2018 John Wiley & Sons Ltd In Southeast Asia, a new disease called scale drop disease (SDD) caused by a novel Megalocytivirus (SDDV) has emerged in farmed Asian sea bass (Lates calcarifer) in Singapore, Malaysia and Indonesia. We received samples from an Eastern Thai province that also showed gross signs of SDD (loss of scales). Clinical samples of 0.2–1.1 kg L. calcarifer collected between 2016 and 2018 were examined for evidence of SDDV infection. Histopathology was similar to that in the first report of SDDV from Singapore including necrosis, inflammation and nuclear pyknosis and karyorrhexis in the multiple organs. Intracytoplasmic inclusion bodies were also observed in the muscle tissue. In a density-gradient fraction from muscle extracts, TEM revealed enveloped, hexagonal megalocytiviral-like particles (~100–180 nm). By PCR using primers derived from the Singaporean SDDV genome sequence, four different genes were amplified and sequenced from the Thai isolate revealing 98.7%–99.9% identity between the two isolates. Since viral inclusions were rarely observed, clinical signs and histopathology could not be used to easily distinguish between SDD caused by bacteria or SDDV. We therefore recommend that PCR screening be used to monitor broodstock, fry and grow-out fish to estimate the current impact of SDDV in Southeast Asia and to prevent its spread.
ISSN: 13652761
Appears in Collections:Scopus 2019

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