Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: Galactomannan pentasaccharide produced from copra meal enhances tight junction integration of epithelial tissue through activation of AMPK
Authors: Chatchai Nopvichai
Pawin Pongkorpsakol
Preedajit Wongkrasant
Karan Wangpaiboon
Thanapon Charoenwongpaiboon
Kazuo Ito
Chatchai Muanprasat
Rath Pichyangkura
Chulalongkorn University
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Osaka City University
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Dec-2019
Citation: Biomedicines. Vol.7, No.4 (2019)
Abstract: © 2019 by the authors. Mannan oligosaccharide (MOS) is well-known as an effective fed supplement for livestock to increase their nutrients absorption and health status. Pentasaccharide of mannan (MOS5) was reported as a molecule that possesses the ability to increase tight junction of epithelial tissue, but the structure and mechanism of action remains undetermined. In this study, the mechanism of action and structure of MOS5 were investigated. T84 cells were cultured and treated with MOS5 compared with vehicle and compound C, a 50-adenosine monophosphate-activated protein kinase (AMPK) inhibitor. The results demonstrated that the ability of MOS5 to increase tight junction integration was inhibited in the presence of dorsomorphine (compound C). Phosphorylation level of AMPK was elevated in MOS5 treated group as determined by Western blot analysis. Determination of MOS5 structure was performed using enzymatic mapping together with 1H, 13C NMR, and 2D-NMR analysis. The results demonstrated that the structure of MOS5 is a β-(1,4)-mannotetraose with α-(1,6)-galactose attached at the second mannose unit from non-reducing end.
ISSN: 22279059
Appears in Collections:Scopus 2019

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.