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dc.contributor.authorAdeline C.Y. Chuaen_US
dc.contributor.authorAbhishek Ananthanarayananen_US
dc.contributor.authorJessica Jie Ying Ongen_US
dc.contributor.authorJen Yi Wongen_US
dc.contributor.authorAndy Yipen_US
dc.contributor.authorNisha Hari Singhen_US
dc.contributor.authorYinghua Quen_US
dc.contributor.authorLaurent Dembeleen_US
dc.contributor.authorMichael McMillianen_US
dc.contributor.authorRatawan Ubaleeen_US
dc.contributor.authorSilas Davidsonen_US
dc.contributor.authorAnchalee Tungtaengen_US
dc.contributor.authorRawiwan Imerbsinen_US
dc.contributor.authorKapish Guptaen_US
dc.contributor.authorChiara Andolinaen_US
dc.contributor.authorFan Leeen_US
dc.contributor.authorKevin S-W Tanen_US
dc.contributor.authorFrançois Nostenen_US
dc.contributor.authorBruce Russellen_US
dc.contributor.authorAmber Langeen_US
dc.contributor.authorThierry T. Diaganaen_US
dc.contributor.authorLaurent Réniaen_US
dc.contributor.authorBryan K.S. Yeungen_US
dc.contributor.authorHanry Yuen_US
dc.contributor.authorPablo Bifanien_US
dc.contributor.otherA-Star, Singapore Immunology Networken_US
dc.contributor.otherMechanobiology Institute, Singaporeen_US
dc.contributor.otherYong Loo Lin School of Medicineen_US
dc.contributor.otherA-Star, Institute of Bioengineering and Nanotechnologyen_US
dc.contributor.otherNovartis Institute for Tropical Diseases Pte. Ltd.en_US
dc.contributor.otherUniversity of Otagoen_US
dc.contributor.otherArmed Forces Research Institute of Medical Sciences, Thailanden_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherNuffield Department of Clinical Medicineen_US
dc.contributor.otherNovartis Institutes for BioMedical Research, Inc.en_US
dc.contributor.otherInvitrocue Pte Ltd. 138667en_US
dc.contributor.otherUniversité des Sciencesen_US
dc.identifier.citationBiomaterials. Vol.216, (2019)en_US
dc.description.abstract© 2019 The Authors Hypnozoites are the liver stage non-dividing form of the malaria parasite that are responsible for relapse and acts as a natural reservoir for human malaria Plasmodium vivax and P. ovale as well as a phylogenetically related simian malaria P. cynomolgi. Our understanding of hypnozoite biology remains limited due to the technical challenge of requiring the use of primary hepatocytes and the lack of robust and predictive in vitro models. In this study, we developed a malaria liver stage model using 3D spheroid-cultured primary hepatocytes. The infection of primary hepatocytes in suspension led to increased infectivity of both P. cynomolgi and P. vivax infections. We demonstrated that this hepatic spheroid model was capable of maintaining long term viability, hepatocyte specific functions and cell polarity which enhanced permissiveness and thus, permitting for the complete development of both P. cynomolgi and P. vivax liver stage parasites in the infected spheroids. The model described here was able to capture the full liver stage cycle starting with sporozoites and ending in the release of hepatic merozoites capable of invading simian erythrocytes in vitro. Finally, we showed that this system can be used for compound screening to discriminate between causal prophylactic and cidal antimalarials activity in vitro for relapsing malaria.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemical Engineeringen_US
dc.subjectMaterials Scienceen_US
dc.titleHepatic spheroids used as an in vitro model to study malaria relapseen_US
Appears in Collections:Scopus 2019

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