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Title: Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor
Authors: Jantana Kampeera
Patiya Pasakon
Chanpen Karuwan
Narong Arunrut
Assawapong Sappat
Sarawut Sirithammajak
Nipaphorn Dechokiattawan
Thitinun Sumranwanich
Parin Chaivisuthangkura
Puey Ounjai
Surang Chankhamhaengdecha
Anurat Wisitsoraat
Adisorn Tuantranont
Wansika Kiatpathomchai
Thailand Ministry of Education
Mahidol University
Thailand National Center for Genetic Engineering and Biotechnology
Thailand National Science and Technology Development Agency
Srinakharinwirot University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Engineering
Issue Date: 1-May-2019
Citation: Biosensors and Bioelectronics. Vol.132, (2019), 271-278
Abstract: © 2019 Elsevier B.V. Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemolyticus by combining loop-mediated isothermal amplification (LAMP) and disposable electrochemical sensors based on screen-printed graphene electrodes (SPGEs). The LAMP reactions using primers targeting V. parahaemolyticus toxR gene were optimized at an isothermal temperature of 65 °C, providing specific detection of V. parahaemolyticus within 45 min at the detection limit of 0.3 CFU per 25 g of raw seafood. The LAMP amplicons can be effectively detected using unmodified SPGEs, redox active molecules namely Hoechst-33258 and a portable potentiostat. Therefore, the proposed system is particularly suitable as a point-of-care device for on-site detection of foodborne pathogens.
ISSN: 18734235
Appears in Collections:Scopus 2019

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