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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/50265
Title: Dengue virus requires apoptosis linked gene-2-interacting protein X (ALIX) for viral propagation
Authors: Chutima Thepparit
Sarawut Khongwichit
Kunjimas Ketsuwan
Sirikwan Libsittikul
Prasert Auewarakul
Duncan R. Smith
Mahidol University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology;Immunology and Microbiology;Medicine
Issue Date: 1-Feb-2019
Citation: Virus Research. Vol.261, (2019), 65-71
Abstract: © 2018 Elsevier B.V. The endosomal sorting complexes required for transport (ESCRT) pathway accessory protein apoptosis linked gene-2-interacting protein X (ALIX) has been shown to be upregulated during dengue virus (DENV) replication. Yeast-two-hybrid screens have additionally shown that ALIX interacts with DENV NS3 protein, but evaluation of the interaction through a replicon assay failed to show a functional significance to the interaction. In this study the interaction between DENV NS3 and ALIX was investigated by co-immunoprecipitation, and functional significance assessed by investigation of DENV production in ALIX expression regulated cells. The results showed that ALIX both interacted and co-localized with DENV NS3 protein and that upregulation of ALIX resulted in a significantly increased viral titer, while either siRNA or CRISPR-Cas9 mediated down regulation of ALIX significantly reduced viral production, without affecting relative DENV genome levels. These results are consistent with ALIX playing a significant role in the DENV replication cycle either during late infection or at viral egress.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/50265
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85059332604&origin=inward
ISSN: 18727492
01681702
Appears in Collections:Scopus 2019

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