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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/50297
Title: Isodon rugosus (Wall. ex Benth.) codd in vitro cultures: Establishment, phytochemical characterization and in vitro antioxidant and anti-aging activities
Authors: Bilal Haider Abbasi
Aisha Siddiquah
Duangjai Tungmunnithum
Shankhamala Bose
Muhammad Younas
Laurine Garros
Samantha Drouet
Nathalie Giglioli-Guivarc’h
Christophe Hano
Quaid-i-Azam University
Université de Tours
Universite d'Orleans
Mahidol University
CNRS Centre National de la Recherche Scientifique
Keywords: Biochemistry, Genetics and Molecular Biology;Chemical Engineering;Chemistry;Computer Science
Issue Date: 2-Jan-2019
Citation: International Journal of Molecular Sciences. Vol.20, No.2 (2019)
Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Isodon rugosus (Wall. ex Benth.) Codd accumulates large amounts of phenolics and pentacyclic triterpenes. The present study deals with the in vitro callus induction from stem and leaf explants of I. rugosus under various plant growth regulators (PGRs) for the production of antioxidant and anti-ageing compounds. Among all the tested PGRs, thidiazuron (TDZ) used alone or in conjunction with α-napthalene acetic acid (NAA) induced highest callogenesis in stem-derived explants, as compared to leaf-derived explants. Stem-derived callus culture displayed maximum total phenolic content and antioxidant activity under optimum hormonal combination (3.0 mg/L TDZ + 1.0 mg/L NAA). HPLC analysis revealed the presence of plectranthoic acid (373.92 µg/g DW), oleanolic acid (287.58 µg/g DW), betulinic acid (90.51 µg/g DW), caffeic acid (91.71 µg/g DW), and rosmarinic acid (1732.61 µg/g DW). Complete antioxidant and anti-aging potential of extracts with very contrasting phytochemical profiles were investigated. Correlation analyses revealed rosmarinic acid as the main contributor for antioxidant activity and anti-aging hyaluronidase, advance glycation end-products inhibitions and SIRT1 activation, whereas, pentacyclic triterpenoids were correlated with elastase, collagenase, and tyrosinase inhibitions. Altogether, these results clearly evidenced the great valorization potential of I. rugosus calli for the production of antioxidant and anti-aging bioactive extracts for cosmetic applications.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/50297
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85060401301&origin=inward
ISSN: 14220067
16616596
Appears in Collections:Scopus 2019

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