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Title: Detection of diverse wolbachia 16s rrna sequences at low titers from malaria vectors in kayin state, myanmar [version 4; peer review: 2 approved, 1 approved with reservations]
Authors: Sunisa Sawasdichai
Victor Chaumeau
Tee Dah
Thithiworada Kulabkeeree
Ladda Kajeechiwa
Monthicha Phanaphadungtham
Muesuwa Trakoolchengkaew
Praphan Kittiphanakun
Yanada Akararungrot
Kyi Oo
Gilles Delmas
Nicholas J. White
François H. Nosten
Mahidol University
Nuffield Department of Clinical Medicine
Keywords: Biochemistry, Genetics and Molecular Biology;Medicine
Issue Date: 1-Jan-2019
Citation: Wellcome Open Research. Vol.4, (2019)
Abstract: � 2019 Sawasdichai S et al. Background: Natural Wolbachia infections in malaria mosquitoes were recently reported in Africa, and negatively correlated with the development of Plasmodium falciparum in the vectors. The occurrence and effects of Wolbachia infections outside Africa have not been described and may have been underestimated. Methods: Mosquitoes were collected by human-landing catch during May and June 2017 in ten villages in Kayin state, Myanmar. Closely related species of malaria vectors were identified with molecular assays. 16S rRNA Wolbachia DNA sequences were detected with quantitative real-time PCR. Results: Low titer of Wolbachia DNA was detected in 13/370 samples in six malaria vector species. Sequences were diverse and different from those described in the African malaria mosquitoes. Conclusion: The detection of Wolbachia DNA in malaria mosquitoes from Kayin state warrants further investigations to understand better the ecology and biology of Anopheles-Wolbachia interactions in Southeast Asia.
ISSN: 2398502X
Appears in Collections:Scopus 2019

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