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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/50862
Title: Detection of human immunoglobulin G by transmission surface plasmon resonance using the in situ gold nanoparticle growth method
Authors: Theerasak Juagwon
Chutiparn Lertvachirapaiboon
Kazunari Shinbo
Keizo Kato
Toemsak Srikhirin
Tanakorn Osotchan
Akira Baba
Niigata University
Mahidol University
Keywords: Engineering;Materials Science
Issue Date: 1-Feb-2019
Citation: IEICE Transactions on Electronics. Vol.E102C, No.2 (2019), 125-131
Abstract: Copyright © 2019 The Institute of Electronics, Information and Communication Engineers. In this work, we report the in situ growth of gold nanoparticles (AuNPs) for the improvement of a transmission surface plasmon resonance (T-SPR) sensor to detect human immunoglobulin G (IgG). Human IgG was immobilized on an activated self-assembled monolayer of 11-mercaptoundecanoic on a gold-coated grating substrate. The T-SPR system was also used to monitor the construction of sensor chips as well as the binding of IgG and anti-IgG conjugated with AuNPs. After specific adsorption with IgG, the T-SPR signal was further enhanced by the in situ growth of AuNPs bound with anti-IgG. Using AuNP conjugation and in situ growth of bound AuNPs, the sensitivity of the IgG immunosensor was improved by two orders of magnitude compared with that without conjugated AuNPs.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/50862
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85061122639&origin=inward
ISSN: 17451353
09168524
Appears in Collections:Scopus 2019

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