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Title: Cadmium induces apoptotic program imbalance and cell cycle inhibitor expression in cultured human astrocytes
Authors: Dusadee Ospondpant
Suttinee Phuagkhaopong
Kran Suknuntha
Kant Sangpairoj
Thitima Kasemsuk
Chutima Srimaroeng
Pornpun Vivithanaporn
University of Wisconsin School of Medicine and Public Health
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Faculty of Medicine, Thammasat University
Mahidol University
Burapha University
Chiang Mai University
Keywords: Environmental Science
Issue Date: 1-Jan-2019
Citation: Environmental Toxicology and Pharmacology. Vol.65, (2019), 53-59
Abstract: © 2018 Elsevier B.V. Cadmium is a highly neurotoxic heavy metal impairing neurogenesis and induces neurodegenerative disorders. Toxic concentrations of cadmium induce astrocytic apoptosis by depleting intracellular glutathione levels, elevating intracellular calcium levels, altering mitochondria membrane potentials, and activating JNK and PI3K/Akt signaling pathways. Cadmium suppresses cell proliferation in kidney epithelial cells, lung fibroblasts, and primary myelocytes; however, cadmium's effects on proteins regulating oxidative stress, apoptosis, and cell proliferation in astrocytes are less known. The present study hypothesized that cadmium alters levels of antioxidant enzymes, apoptotic regulator proteins, and cell cycle inhibitor proteins, resulting in apoptosis and cell cycle arrest. Concentrations ≥20 μM cadmium induced apoptosis and led to intracellular changes including DNA fragmentation, reduced mRNA expression of antioxidant enzymes (i.e., catalase and glutathione S transferase-A4), downregulation of B-cell lymphoma 2 (Bcl-2), and upregulation of Bcl-2-associated X protein (Bax). Moreover, cadmium suppressed astrocytic proliferation by inducing S and G2/M phase cell cycle arrest and promoting p53, p21, and p27 expression. In conclusion, this study provides mechanistic insight into cadmium-induced cytotoxicity of astrocytes and highlights potential targets for prevention of cadmium-induced apoptosis and cell cycle arrest.
ISSN: 18727077
Appears in Collections:Scopus 2019

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