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Title: Determination of cell expansion and surface molecule expression on anti-CD3/28 expanded CD4<sup>+</sup> T cells
Authors: Premrutai Thitilertdecha
Poonsin Poungpairoj
Varangkana Tantithavorn
Palanee Ammaranond
Nattawat Onlamoon
Chulalongkorn University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Immunology and Microbiology
Issue Date: 1-Nov-2019
Citation: Scandinavian Journal of Immunology. Vol.90, No.5 (2019)
Abstract: © 2019 The Scandinavian Foundation for Immunology CD4+ T cell immunotherapy has potential for treatment in HIV-infected patients. A large number of expanded CD4+ T cells and confirmation of functional-related phenotypes are required for ensuring the successful outcomes of treatment. Freshly isolated CD4+ T cells from healthy donors were activated with anti-CD3/28-coated magnetic beads at different bead-to-cell ratios and cultured in the absence and presence of IL-2 supplementation for 3 weeks. Fold expansion, cell viability, growth kinetic and lymphocyte subset identities were determined. Data demonstrated that a 1:1 bead-to-cell ratio rendered the highest expansion of 1044-fold with 88% viability and 99.5% purity followed by the 2:1 and 0.5:1 ratios. No significant difference in proliferation and phenotypes was found between non–IL-2 and IL-2 supplementation groups. Several specific surface molecule expressions of the expanded cells including chemokine receptors, adhesion molecules, co-stimulatory molecules, activation molecules, maturation markers, cytokine receptors and other molecules were altered when compared to the unexpanded cells. This optimized expansion protocol using the 1:1 bead-to-cell ratio of anti-CD3/28-coated magnetic beads and culture condition without IL-2 supplementation provided the satisfactory yield with good reproducibility. Specific surface molecule expressions of the expanded cells presented potential roles in proliferation, differentiation, homeostasis, apoptosis and organ homing.
ISSN: 13653083
Appears in Collections:Scopus 2019

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