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|Title:||Remodeling of the actin network associated with the non-structural protein 1 (NS1) of west Nile virus and formation of NS1-containing tunneling nanotubes|
Marie Pierre Confort
Saw See Hong
University of Phayao
École Pratique des Hautes Etudes
Université Claude Bernard Lyon 1
Ecole Nationale Vétérinaire d'Alfort
CNRS Centre National de la Recherche Scientifique
|Keywords:||Immunology and Microbiology;Medicine|
|Citation:||Viruses. Vol.11, No.10 (2019)|
|Abstract:||© 2019 by the authors. Licensee MDPI, Basel, Switzerland. The cellular response to the recombinant NS1 protein of West Nile virus (NS1WNV) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of NS1WNV: (i) the exogenous secreted form, sNS1WNV, added to the extracellular milieu; and (ii) the endogenous NS1WNV, the intracellular form expressed in plasmid-transfected cells. The cell attachment and uptake of sNS1WNV varied with the cell type and were only detectable in Vero E6 and SH-SY5Y cells. Addition of sNS1WNV to the cell culture medium resulted in significant remodeling of the actin filament network in Vero E6 cells. This effect was not observed in SH-SY5Y and U-87MG cells, implying that the cellular uptake of sNS1WNV and actin network remodeling were dependent on cell type. In the three cell types, NS1WNV-expressing cells formed filamentous projections reminiscent of tunneling nanotubes (TNTs). These TNT-like projections were found to contain actin and NS1WNV proteins. Interestingly, similar actin-rich, TNT-like filaments containing NS1WNV and the viral envelope glycoprotein EWNV were also observed in WNV-infected Vero E6 cells.|
|Appears in Collections:||Scopus 2019|
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