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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/51017
Title: Remodeling of the actin network associated with the non-structural protein 1 (NS1) of west Nile virus and formation of NS1-containing tunneling nanotubes
Authors: Wilhelm Furnon
Pascal Fender
Marie Pierre Confort
Sophie Desloire
Sawitree Nangola
Kuntida Kitidee
Caroline Leroux
Maxime Ratinier
Frédérick Arnaud
Sylvie Lecollinet
Pierre Boulanger
Saw See Hong
University of Phayao
École Pratique des Hautes Etudes
Université Claude Bernard Lyon 1
Mahidol University
Ecole Nationale Vétérinaire d'Alfort
CNRS Centre National de la Recherche Scientifique
Inserm
Keywords: Immunology and Microbiology;Medicine
Issue Date: 27-Sep-2019
Citation: Viruses. Vol.11, No.10 (2019)
Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. The cellular response to the recombinant NS1 protein of West Nile virus (NS1WNV) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of NS1WNV: (i) the exogenous secreted form, sNS1WNV, added to the extracellular milieu; and (ii) the endogenous NS1WNV, the intracellular form expressed in plasmid-transfected cells. The cell attachment and uptake of sNS1WNV varied with the cell type and were only detectable in Vero E6 and SH-SY5Y cells. Addition of sNS1WNV to the cell culture medium resulted in significant remodeling of the actin filament network in Vero E6 cells. This effect was not observed in SH-SY5Y and U-87MG cells, implying that the cellular uptake of sNS1WNV and actin network remodeling were dependent on cell type. In the three cell types, NS1WNV-expressing cells formed filamentous projections reminiscent of tunneling nanotubes (TNTs). These TNT-like projections were found to contain actin and NS1WNV proteins. Interestingly, similar actin-rich, TNT-like filaments containing NS1WNV and the viral envelope glycoprotein EWNV were also observed in WNV-infected Vero E6 cells.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/51017
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85072783021&origin=inward
ISSN: 19994915
Appears in Collections:Scopus 2019

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