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dc.contributor.authorWilhelm Furnonen_US
dc.contributor.authorPascal Fenderen_US
dc.contributor.authorMarie Pierre Conforten_US
dc.contributor.authorSophie Desloireen_US
dc.contributor.authorSawitree Nangolaen_US
dc.contributor.authorKuntida Kitideeen_US
dc.contributor.authorCaroline Lerouxen_US
dc.contributor.authorMaxime Ratinieren_US
dc.contributor.authorFrédérick Arnauden_US
dc.contributor.authorSylvie Lecollineten_US
dc.contributor.authorPierre Boulangeren_US
dc.contributor.authorSaw See Hongen_US
dc.contributor.otherUniversity of Phayaoen_US
dc.contributor.otherÉcole Pratique des Hautes Etudesen_US
dc.contributor.otherUniversité Claude Bernard Lyon 1en_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherEcole Nationale Vétérinaire d'Alforten_US
dc.contributor.otherCNRS Centre National de la Recherche Scientifiqueen_US
dc.identifier.citationViruses. Vol.11, No.10 (2019)en_US
dc.description.abstract© 2019 by the authors. Licensee MDPI, Basel, Switzerland. The cellular response to the recombinant NS1 protein of West Nile virus (NS1WNV) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma cells, and U-87MG human astrocytoma cells. Cells were exposed to two different forms of NS1WNV: (i) the exogenous secreted form, sNS1WNV, added to the extracellular milieu; and (ii) the endogenous NS1WNV, the intracellular form expressed in plasmid-transfected cells. The cell attachment and uptake of sNS1WNV varied with the cell type and were only detectable in Vero E6 and SH-SY5Y cells. Addition of sNS1WNV to the cell culture medium resulted in significant remodeling of the actin filament network in Vero E6 cells. This effect was not observed in SH-SY5Y and U-87MG cells, implying that the cellular uptake of sNS1WNV and actin network remodeling were dependent on cell type. In the three cell types, NS1WNV-expressing cells formed filamentous projections reminiscent of tunneling nanotubes (TNTs). These TNT-like projections were found to contain actin and NS1WNV proteins. Interestingly, similar actin-rich, TNT-like filaments containing NS1WNV and the viral envelope glycoprotein EWNV were also observed in WNV-infected Vero E6 cells.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleRemodeling of the actin network associated with the non-structural protein 1 (NS1) of west Nile virus and formation of NS1-containing tunneling nanotubesen_US
Appears in Collections:Scopus 2019

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