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dc.contributor.authorAchinya Phuakroden_US
dc.contributor.authorWitsaroot Sripumkhaien_US
dc.contributor.authorWutthinan Jeamsaksirien_US
dc.contributor.authorPattaraluck Pattamangen_US
dc.contributor.authorEkachai Juntasaroen_US
dc.contributor.authorTherdthai Thienthongen_US
dc.contributor.authorSuporn Foongladdaen_US
dc.contributor.authorPaul J. Brindleyen_US
dc.contributor.authorSirichit Wongkamchaien_US
dc.contributor.otherKing Mongkut's University of Technology North Bangkoken_US
dc.contributor.otherThailand National Electronics and Computer Technology Centeren_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.contributor.otherThe George Washington Universityen_US
dc.identifier.citationParasites and Vectors. Vol.12, No.1 (2019)en_US
dc.description.abstract© 2019 The Author(s). Background: The diagnosis of filariasis traditionally relies on the detection of circulating microfilariae (mf) using Giemsa-stained thick blood smears. This approach has several limitations. We developed a semi-automated microfluidic device to improve and simplify the detection of filarial nematodes. Methods: The efficiency and repeatability of the microfluidic device was evaluated. Human EDTA blood samples were 'spiked' with B. malayi mf at high, moderate, and low levels, and subsequently tested 10 times. The device was also used for a field survey of feline filariasis in 383 domesticated cats in an area of Narathiwat Province, Thailand, the endemic area of Brugia malayi infection. Results: In the control blood arbitrarily spiked with mf, the high level, moderate level and low level mf-positive controls yielded coefficient variation (CV) values of 4.44, 4.16 and 4.66%, respectively, at the optimized flow rate of 6 μl/min. During the field survey of feline filariasis in Narathiwat Province, the device detected mf in the blood of 34 of 383 cats (8.9%) whereas mf were detected in 28 (7.3%) cats using the blood smear test. Genomic DNA was extracted from mf trapped in the device after which high-resolution melting (HRM) real-time PCR assay was carried out, which enabled the simultaneous diagnosis of filarial species. Among the 34 mf-positive samples, 12 were identified as B. malayi, 15 as Dirofilaria immitis and 7 as| D. repens. Conclusions: We developed a semi-automated microfluidic device to detect mf of filarial parasites that could be used to diagnose lymphatic filariasis in human populations. This novel device facilitates rapid, higher-throughput detection and identification of infection with filariae in blood samples.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunology and Microbiologyen_US
dc.titleDiagnosis of feline filariasis assisted by a novel semi-automated microfluidic device in combination with high resolution melting real-time PCRen_US
Appears in Collections:Scopus 2019

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