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Title: Novel differential linear B-cell epitopes to identify Zika and dengue virus infections in patients
Authors: Siti Naqiah Amrun
Wearn Xin Yee
Farhana Abu Bakar
Bernett Lee
Yiu Wing Kam
Fok Moon Lum
Jeslin J.L. Tan
Vanessa W.X. Lim
Wanitda Watthanaworawit
Clare Ling
Francois Nosten
Laurent Renia
Yee Sin Leo
Lisa F.P. Ng
A-Star, Singapore Immunology Network
Yong Loo Lin School of Medicine
University of Liverpool
National University of Singapore
Mahidol University
Nuffield Department of Clinical Medicine
National Institutes of Health, Bethesda
Nanyang Technological University
Tan Tock Seng Hospital
Keywords: Immunology and Microbiology
Issue Date: 1-Jan-2019
Citation: Clinical and Translational Immunology. Vol.8, No.7 (2019)
Abstract: © 2019 The Authors. Clinical & Translational Immunology published by John Wiley & Sons Australia, Ltd on behalf of Australian and New Zealand Society for Immunology Inc. Objectives: Recent Zika virus (ZIKV) outbreaks challenged existing laboratory diagnostic standards, especially for serology-based methods. Because of the genetic and structural similarity of ZIKV with other flaviviruses, this results in cross-reactive antibodies, which confounds serological interpretations. Methods: Plasma from Singapore ZIKV patients was screened longitudinally for antibody responses and neutralising capacities against ZIKV. Samples from healthy controls, ZIKV patients and DENV patients were further assessed using ZIKV and DENV peptides of precursor membrane (prM), envelope (E) or non-structural 1 (NS1) viral proteins in a peptide-based ELISA for epitope identification. Identified epitopes were re-validated and diagnostically evaluated using sera of patients with DENV, bacteria or unknown infections from Thailand. Results: Long-lasting ZIKV-neutralising antibodies were elicited during ZIKV infection. Thirteen potential linear B-cell epitopes were identified, and of these, four common flavivirus, three ZIKV-specific and one DENV-specific differential epitopes had more than 50% sensitivity and specificity. Notably, ZIKV-specific peptide 26 on domain I/II of E protein (amino acid residues 271–288) presented 80% sensitivity and 85.7% specificity. Importantly, the differential epitopes also showed significance in differentiating non-flavivirus patient samples. Conclusion: Linear B-cell epitope candidates to differentiate between ZIKV and DENV infections were identified, providing the first step towards the design of a much-needed serology-based assay.
ISSN: 20500068
Appears in Collections:Scopus 2019

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