Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: Cytotoxicity of an experimental light-cured orthodontic adhesive
Authors: Kanin Nimcharoensuk
Niwat Anuwongnukroh
Surachai Dechkunakorn
Vanthana Sattabanasuk
Panya Sunintaboon
Wassana Wichai
Mahidol University
Keywords: Materials Science
Issue Date: 1-Jan-2019
Citation: Solid State Phenomena. Vol.294 SSP, (2019), 65-70
Abstract: © 2019 Trans Tech Publications Ltd, Switzerland. The objective of this study was to compare the cytotoxicity of a domestically-made light-cured orthodontic adhesive to a commercial adhesive, Transbond XT (3M Unitek, USA). An in-house orthodontic adhesive composed of a filler 60-70 weight % and a monomer ratio (BisGMA:TEGDMA) of 6:4 with 0.5% of photoinitiator was mixed. The potential cytotoxic effect of this experimental and a control adhesive was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay according to ISO 10993-5: 2009(E). The L929 cell line was grown in 96-well tissue culture plates (1x105 cells/mm3 ). Thin cured-resin discs of each material weighing 0.4 gram were prepared and incubated for 1, 3, 5, 7, 14, and 30 days in Dulbecco’s modified Eagle medium (DMEM) at 37˚C and 95% humidity with 5% CO2. The percentage of cell viability was reported by descriptive statistics. The result showed that the cell viability of the experimental adhesive was higher than Transbond XT in all measured periods. The cytotoxicity of both the adhesives gradually decreased with the progression of time. In conclusion, the in-house adhesive showed a good biocompatibility since the first day following polymerization. On the other hand, Transbond XT started with a cytotoxic potential, then, turned to be non-cytotoxic after 5 days of curing.
ISSN: 16629779
Appears in Collections:Scopus 2019

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.